Abstract
AXONAL elongation and the transformation of growth cones to synaptic terminals are major steps of brain development and the molecular mechanisms involved form the basis of the correct wiring of the nervous system. The same mechanisms may also contribute to the remodelling of nerve terminals that occurs in the adult brain, as a morphological substrate to memory and learning1. We have investigated the function of the nerve terminal protein SNAP-25 (ref. 2) during development. We report here that SNAP-25 is expressed in axonal growth cones during late stages of elongation and that selective inhibition of SNAP-25 expression prevents neurite elongation by rat cortical neurons and PC-12 cells in vitro and by amacrine cells of the developing chick retina in vivo. These results demonstrate that SNAP-25 plays a key role in axonal growth. They also suggest that high levels of SNAP-25 expression in specific areas of the adult brain2 may contribute to nerve terminal plasticity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Wallace, S. W. et al. in Long-Term Potentiation: a Debate of Current Issues (eds Baudry, M. & Davis, J. L.) 189–232 (MIT press, Cambridge, MA 1991).
Oyler, G. A. et al. J. Cell Biol. 109, 3039–3052 (1989).
Catsicas, S. et al. J. Neurosci. Res. 33, 1–9 (1992).
Loewy, A. et al. J. Neurosci. 11, 3412–3421 (1991).
Hess, D. T. et al. J. Neurosci. 12, 4634–4641 (1992).
Catsicas, S. et al. Proc. nat. Acad. Sci. U.S.A. 88, 785–789 (1991).
Oyler, G. A. et al. Proc. nat. Acad. Sci. U.S.A. 88, 5247–5251 (1991).
Oyler, G. A. et al. Dev. Brain Res. 65, 133–146 (1992).
Bark, C. & Wilson, M. C. Abstr. Soc. Neurosci. 17 531 (1991).
Alvarez-Bolado, G. et al. Abstr. Soc. Neurosci. 17, 215 (1991).
Sudhof, T. C. et al. Science 238, 1142–1144 (1987).
Greene, L. A. & Tischler, A. S. Proc. nat. Acad. Sci. U.S.A. 73, 2424–2428 (1976).
Sanna, P. P., Bloom, F. E. & Wilson, M. C. Dev Brain Res. 59, 104–108 (1991).
Gunning, P. W. et al. J. Cell Biol. 89, 240–245 (1981).
Celio, M. R. Neuroscience 35, 375–475 (1990).
Ellis, J. H., Richards, D. E. & Rogers, J. H. Cell Tissue Res. 264, 197–208 (1991).
Masliah, E. et al. J. Histochem. Cytochem. 38, 837–844 (1990).
Geddes, J. W. et al. Neuroscience 38, 515–525 (1990).
Hess, E. J. et al. J. Neurosci. 12, 2865–2874 (1992).
Söllner, T. et al. Nature 362, 318–324 (1993).
Pfenninger, K. H. et al. in The Growth Cone (eds Letourneau, P. C., Kater, S. B. & Macagno, E. R.) 111–123 (Raven, New York, 1991).
Baughman, R. W. et al. in Culturing nerve cells (eds Banker, G. & Goslin, K.) 227–249 (MIT Press, Cambridge, MA, 1991).
Catsicas, S. & Clarke, P. G. H. J. comp. Neurol. 262, 512–522 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Osen-Sand, A., Catsicas, M., Staple, J. et al. Inhibition of axonal growth by SNAP-25 antisense oligonucleotides in vitro and in vivo. Nature 364, 445–448 (1993). https://doi.org/10.1038/364445a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/364445a0
This article is cited by
-
Effect of short-term oral supplementation with marine-derived n-3 polyunsaturated fatty acids on gene expression of synaptosome-associated protein 25 in the olfactory organ and brain of juvenile chum salmon Oncorhynchus keta
Fisheries Science (2024)
-
Delayed Outgrowth in Response to the BDNF and Altered Synaptic Proteins in Neurons From SHR Rats
Neurochemical Research (2023)
-
KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization
Communications Biology (2022)
-
Synaptotagmin 13 is neuroprotective across motor neuron diseases
Acta Neuropathologica (2020)
-
The Association of SNAP25 Gene Polymorphisms in Attention Deficit/Hyperactivity Disorder: a Systematic Review and Meta-Analysis
Molecular Neurobiology (2017)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
