Abstract
NEUTROPHIL recruitment into tissues is a multistep process involving sequential engagement of adhesion molecules, including selectins (E,P,L), which are reactive with oligosaccharides, and the family of (β2 integrins which are reactive with endothelial intercellular adhesion molecules1–3. These processes result in the initial rolling of leukocytes along the endothelial surfaces, followed by the firm attachment of leukocytes to the endothelium. The intravenous infusion of cobra venom factor into rats results in acute lung injury that is neutrophil-dependent, oxygen radical mediated and P-selectin-dependent4,5. Here we report that infusion of sialyl-Lewis X, a ligand for P-selectin6–9, dramatically reduced lung injury and diminished the tissue accumulation of neutrophils, whereas irrelevant oligosaccharides had no such effects. These results suggest that sialyl-Lewis X carbohydrates may be used as a new strategy for anti-inflammatory therapy.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Butcher, D. C. Cell 67, 1033–1036 (1991).
Lawrence, M. B. & Springer, T. A. Cell 65, 859–873 (1991).
Lorant, D. E. et al. J. Cell Biol. 115, 223–234 (1991).
Till, G. O. Johnson, K. J., Kunkel, R. & Ward, P. A. J. clin. Invest. 69, 1126–1135 (1982).
Mulligan, M. S. et al. J. clin. Invest. 90, 1600–1607 (1992).
Larsen, E. et al. Cell 59, 305–312 (1989).
Geng, J-G. et al. Nature 343, 757–760 (1990).
Polley, M. J. et al. Proc. natn. Acad. Sci. U.S.A. 88, 6224–6228 (1991).
Zhou, Q. et al. J. Cell Biol. 115, 557–564 (1991).
Springer, T. A. & Lasky, L. A. Nature 349, 196–197 (1991).
Paulson, J. C. in Adhesion: Its Role in Inflammatory Disease 19–42 (Freeman, New York, 1992).
Spragg, R. G. & Smith, R. M. in The Lung: Scientific Foundation (eds Crystal, R. G. & West, J. B.) 2003–2017 (Raven, New York, 1991).
Ichikawa, Y. et al. J. am. chem. Soc. (in the press).
Ball, G. E. et al. J. am. chem. Soc. 114, 5449–5451 (1992).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mulligan, M., Paulson, J., Freest, S. et al. Protective effects of oligosaccharides in P-selectin-dependent lung injury. Nature 364, 149–151 (1993). https://doi.org/10.1038/364149a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/364149a0
This article is cited by
-
Regulation of leukocyte recruitment by the long pentraxin PTX3
Nature Immunology (2010)
-
Cell Adhesion Molecules: Potential Therapeutic & Diagnostic Implications
Molecular Biotechnology (2008)
-
Monomeric and Multimeric Blockers of Selectins: Comparison of in vitro and in vivo Activity
Biochemistry (Moscow) (2005)
-
P-selectin in arterial thrombosis
Zeitschrift f�r Kardiologie (2004)
-
A novel non-invasive, in vivo technique for the quantification of leukocyte rolling and extravasation at sites of inflammation in human patients
Nature Medicine (2001)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
