Abstract
TRANSCRIPTIONAL activating sequences have been described1 that are encoded by parts of the genome of Escherichia coli. These acidic peptides, fused to a DNA-binding fragment of the yeast transcriptional activator GAL4, activate transcription of a gene in a wide array of eukaryotes, provided that gene bears GAL4-binding sites nearby2–4. Here we describe an E. coil-encoded sequence that, when attached to the same DNA-binding fragment (GAL4( 1–147)), converts that fragment into a repressor. Thus, as assayed in yeast or in vitro in yeast extracts, this molecule represses transcription when bound upstream of a variety of different activators. Two additional repressing regions that work when tethered upstream, a multiple mutant derivative of the original isolate and a synthetic peptide are, like the original isolate, highly basic. At least one activator can be inhibited by the mutant but not by the parental repressing region. These and other findings suggest that these repressing regions interact with and inhibit the activity of activating regions bound nearby on DNA.
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References
Ma, J. & Ptashne, M. Cell 51, 113–119 (1987).
Ptashne, M. A Genetic Switch: Phage Lambda and Higher Organisms (Cell Press and Blackwell Scientific, Cambridge, MA, 1992).
Ptashne, M. & Gann, A. A. F. Nature 346, 329–331 (1990).
Lewin, B. Cell 61, 1161–1164 (1990).
Hope, I. A. & Struhl, K. Cell 46, 885–894 (1986).
Keleher, C. A., Redd, M. J., Schultz, J., Carlson, M. & Johnson, A. D. Cell 68, 709–719 (1992).
Schultz, J. & Carlson, M. Molec. cell. Biol. 7, 3637–3645 (1987).
Steward, R. Science 238, 692–694 (1987).
Kamens, J. & Brent, R. New Biol. 3, 1005–1013 (1991).
Ray, R. P., Arora, K., Nüsslein-Volhard, C. & Gelbart, W. M. Developement 113, 35–54 (1991).
Jiang, J., Kosman, D., Ip, Y. T. & Levine, M. Genes Dev. 5, 1881–1891 (1991).
Roy, A., Exinger, F. & Losson, R. Molec. cell. Biol. 10, 5257–5270 (1990).
Ruden, D. M., Ma, J., Li, Y., Wood, K. & Ptashne, M. Nature 350, 250–252 (1991).
Han, K. & Manley, J. L. Genes Dev. 7, 491–503 (1993).
Johnson, F. B. & Krasnow, M. A. Genes Dev. 6, 2177–2189 (1992).
Workman, J. L. & Roeder, R. G. Cell 51, 613–622 (1987).
Licht, J. D., Grossel, M. J., Figge, J. & Hansen, U. M. Nature 346, 76–79 (1990).
Sauer, F. & Jäckle, H. Nature 353, 563–566 (1991).
Ma, J. & Ptashne, M. Cell 48, 847–853 (1987).
Devlin, C., Kimberly, T.-B., Shore, D. & Arndt, K. T. Molec. cell. Biol. 11, 3642–3651 (1991).
Lin, Y.-S., Carey, M. F., Ptashne, M. & Green, M. R. Cell 54, 659–664 (1988).
Lue, N. F., Flanagan, P. M., Sugimoto, K. & Kornberg, R. D. Science 246, 661–664 (1989).
Ponticelli, A. S. & Struhl, K. Molec. cell. Biol. 10, 2832–2839 (1990).
van Holde, K. E. Chromatin (Springer, New York, 1989).
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Saha, S., Brickman, J., Lehming, N. et al. New eukaryotic transcriptional repressers. Nature 363, 648–652 (1993). https://doi.org/10.1038/363648a0
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DOI: https://doi.org/10.1038/363648a0
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