Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Product Review
  • Published:

Sequence-specific scission of DNA by RNAs linked to a chemical nuclease

RNAs linked to the chemical nuclease 1,10-phenanthroline-copper cut double-stranded DNA of complementary sequence. This cleavage reaction is applicable to all sequences and can be used to measure the distance between marker genes in base pairs, map the size of a transcription unit and define positions of chromosomal breakpoints.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

References

  1. Chen, C.-h. B., Gorin, M. B. & Sigman, D. S. Proc. natn. Acad. Sci. U.S.A. 90, 4206–4210 (1993).

    Article  ADS  CAS  Google Scholar 

  2. Dervan, P. B. Nature 359, 87–88 (1992).

    Article  ADS  CAS  Google Scholar 

  3. Koob, M. & Szybalski, W. Science 250, 271–273 (1990).

    Article  ADS  CAS  Google Scholar 

  4. Ferrin, L. J. & Camerini-Otero, R. D. Science 254, 1494–1497 (1991).

    Article  ADS  CAS  Google Scholar 

  5. Sigman, D. S. & Chen, C.-h. B. A. Rev. Biochem. 59, 207–236 (1990).

    Article  CAS  Google Scholar 

  6. Sigman, D. S., Graham, D. R., D'Aurora, V. & Stern, A. M. J. biol. Chem. 254, 12269–12272 (1979).

    CAS  PubMed  Google Scholar 

  7. Goyne, T. E. & Sigman, D. S. J. Am. chem. Soc. 109, 2846–2848 (1987).

    Article  CAS  Google Scholar 

  8. Sigman, D. S. Acc. Chem. Res. 19, 180–186 (1986).

    Article  CAS  Google Scholar 

  9. Dervan, P. B. Science 232, 464–471 (1986).

    Article  ADS  CAS  Google Scholar 

  10. Chen, C.-h. B., Mazumder, A., Constant, J.-F. & Sigman, D. S. Bioconjugate Chem. 4, 69–77 (1993).

    Article  CAS  Google Scholar 

  11. Sigman, D. S., Bruice, T. W., Mazumder, A. & Sutton, C. L. Acc. chem. Res. 26, 98–104 (1993).

    Article  CAS  Google Scholar 

  12. Chen, C.-h. B. & Sigman, D. S. Proc. natn. Acad. Sci. U.S.A. 83, 7147–7151 (1986).

    Article  ADS  CAS  Google Scholar 

  13. Chen, C.-h. B. & Sigman, D. S. J. Am. chem. Soc. 110, 6570–6572 (1988).

    Article  CAS  Google Scholar 

  14. Sutton, C., Mazumder, A., Chen, C.-h. B. & Sigman, D. S. Biochemistry 32, 4225–4230 (1993).

    Article  CAS  Google Scholar 

  15. Ebright, R. H., Ebright, Y. W., Pendergast, P. S. & Gunasekera, A. Proc. natn. Acad. Sci. U.S.A. 87, 2882–2886 (1990).

    Article  ADS  CAS  Google Scholar 

  16. Pendergast, P. S., Ebright, Y. W. & Ebright, R. H. J. cell. Biochem. 17, 156 Abs. KO31 (1993).

    Google Scholar 

  17. Bruice, T. W., Wise, J., Rosser, D. S. E. & Sigman, D. S. J. Am. chem. Soc. 113, 5446–5447 (1991).

    Article  CAS  Google Scholar 

  18. Langer, P. R., Waldrop, A. A. & Ward, D. C. Proc. natn. Acad. Sci. U.S.A. 78, 6633–6637 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Thomas, M., White, R. L. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 2294–2298 (1976).

    Article  ADS  CAS  Google Scholar 

  20. Rosbash, M. et al. Meth. Enzym. 68, 454–469 (1979).

    Article  CAS  Google Scholar 

  21. Nickerson, J. M. et al. J. biol. Chem. 260, 9100–9105 (1985).

    CAS  PubMed  Google Scholar 

  22. Nickerson, J. M. et al. J. biol. Chem 261, 552–557 (1986).

    CAS  PubMed  Google Scholar 

  23. Corey, D. R., Pei, D. & Schultz, P. G. Biochemistry 28, 8277–8286 (1989).

    Article  CAS  Google Scholar 

  24. Longmire, J. L., Albright, K. L., Lewis, A. K., Meincke, L. J. & Hildebrand, C. E. Nucleic Acids Res. 15, 859 (1987).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sigman, D., Chen, Ch. & Gorin, M. Sequence-specific scission of DNA by RNAs linked to a chemical nuclease. Nature 363, 474–475 (1993). https://doi.org/10.1038/363474a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/363474a0

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing