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Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins

Nature volume 362, pages 648652 (15 April 1993) | Download Citation

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Abstract

Do the coats on vesicles budded from the Golgi apparatus actually cause the budding, or do they simply coat buds (Fig. 1)? One view (the membrane-mediated budding hypothesis1) is that budding is an intrinsic property of Golgi membranes not requiring extrinsic coat proteins. Assembly of coats from dispersed subunits is superimposed upon the intrinsic budding process and is proposed to convert the tips of tubules into vesicles. The alternative view (the coat-mediated budding hypothesis1) is that coat formation provides the essential driving force for budding. The membrane-mediated budding hypothesis was inspired by the microtubule-dependent extension of apparently uncoated, 90-nm-diameter membrane tubules from the Golgi apparatus2 and other organelles3–5 in vivo after treatment with brefeldin A, a drug that inhibits the assembly of coat proteins onto Golgi membranes6–9. This hypothesis predicts that tubules will be extended when coat proteins are unavailable to convert tubule-derived membrane into vesicles. Here we use a cell-free system in which coated vesicles are formed from Golgi cisternae to show that, on the contrary, when budding diminishes as a result of immunodepletion of coat protein pools, tubules are not formed at the expense of vesicles. We conclude that coat proteins are required for budding from Golgi membranes.

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References

  1. 1.

    , & J. Cell Biol. 116, 1071–1080 (1992).

  2. 2.

    et al. Cell 60, 821–836 (1990).

  3. 3.

    , & Cell 67, 617–628 (1991).

  4. 4.

    et al. Cell 67, 601–616 (1991).

  5. 5.

    , & Cell 67, 691–600 (1991).

  6. 6.

    et al. J. Cell Biol 111, 2295–2306 (1990).

  7. 7.

    et al. Cell 64, 649–665 (1991).

  8. 8.

    et al. Cell 64, 1183–1195 (1991).

  9. 9.

    & Cell 69, 129–138 (1992).

  10. 10.

    et al. Cell 39, 405–416 (1984)

  11. 11.

    , & Cell 46, 171–184 (1986).

  12. 12.

    et al. Cell 51, 1053–1062 (1987).

  13. 13.

    et al. Cell 58, 329–336 (1989).

  14. 14.

    et al. Nature 349, 215–220 (1991).

  15. 15.

    , & Nature 349, 248–251 (1991).

  16. 16.

    et al. J. biol Chem. 266, 2606–2614 (1991).

  17. 17.

    et al. Cell 67, 239–253 (1991).

  18. 18.

    et al. Science 254, 1197–1199 (1991).

  19. 19.

    et al. Proc. natn. Acad. Sci. U.S.A. 89, 6408–6412 (1992).

  20. 20.

    & Nature 360, 352–354 (1992).

  21. 21.

    & Cell 68, 829–840 (1992).

  22. 22.

    Cell 67, 449–451 (1991).

  23. 23.

    & A. Rev Biochem 50, 85–101 (1981).

  24. 24.

    , , & Science 236, 558–563 (1987).

  25. 25.

    , , & J. Cell Biol. 108, 843–853 (1989).

  26. 26.

    et al. Cell 56, 357–368 (1989).

  27. 27.

    , , & J. Cell Biol. 120, 15–24 (1993).

  28. 28.

    & J. Cell Biol. 103, 2229–2239 (1986).

  29. 29.

    , & Meth. Enzym. 219, 331 (1992).

  30. 30.

    , & J. biol. Chem. (in the press).

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Affiliations

  1. Institute of Histology and Embryology, University of Geneva Medical School, 1 rue Michel Servet, 1211 Geneva 4, Switzerland

    • Lelio Orci
    • , Mariella Ravazzola
    • , Alain Perrelet
    •  & Mylène Amherdt
  2. Program in Cellular Biochemistry and Biophysics, Sloan‐Kettering Institute, 1275 York Avenue, New York, New York 10021, USA

    • David J. Palmer
    •  & James E. Rothman

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https://doi.org/10.1038/362648a0

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