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A nutrient-permeable channel on the intraerythrocytic malaria parasite

Nature volume 362, pages 643646 (15 April 1993) | Download Citation

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Abstract

DURING its 48-hour cycle inside the red blood cell, the human malaria parasite, Plasmodium falciparum, increases its volume 25-fold and divides asexually. This rapid growth demands large amounts of nutrients, a problem exacerbated by the lower metabolic rate and relative ionic impermeability of the host red blood cell. Direct passage of small nutrients across the two membranes that separate the parasite from the erythrocyte cytosol may be important for parasite development1 and has been demonstrated for radiolabelled glucose2, amino acids3,4 and purine nucleo-sides4–6. Flux studies on plasmodia are limited, however, to suspensions of erythrocyte-free parasites and so cannot be used to examine the individual transport properties of the two membranes involved. Here we use the cell-attached patch clamp method7 to overcome this limitation. After removing the intervening red blood cell membrane and forming gigaohm seals on the small (3–5 μm) parasite, we studied transport across the parasitophorous vacuole membrane (PVM), the outer of the two membranes that separate the parasite from the erythrocyte cytosol. A 140-pS channel which is permeable to both cations and anions was identified on the PVM. This channel is present at high density, is open more than 98 per cent of the time at the resting potential of the PVM, and is permeable to lysine and glucuronate. The channel can readily transport amino acids and monosaccharides across the PVM and may be essential for fulfilling the parasite's metabolic demands.

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References

  1. 1.

    Parasitology 96, S57–S81 (1988).

  2. 2.

    Parasit. Today 6, 225–229 (1990).

  3. 3.

    J. Protozool. 18, 392–399 (1971).

  4. 4.

    Bull. World Health Org. 55, 211–225 (1977).

  5. 5.

    , & J. Parasit 66, 205–212 (1980).

  6. 6.

    & J. biol. Chem. 264, 3194–3199 (1989).

  7. 7.

    , , , & Pflügers Arch. 391, 85–100 (1981).

  8. 8.

    Bull. World Health Org. 55, 171–178 (1977).

  9. 9.

    & Molec. biochem. Parasit. 3, 253–264 (1981).

  10. 10.

    , , , & J. Cell Biol. 89, 456–474 (1981).

  11. 11.

    , & J. gen. Physiol. 75, 469–492 (1980).

  12. 12.

    & Q. Rev. Biophys. 24, 367–403 (1990).

  13. 13.

    & Nature 327, 522–524 (1987).

  14. 14.

    & Adv. exp. Med. Biol. 309A, 169–172 (1991).

  15. 15.

    Comp. biochem. Physiol. 95A, 31–39 (1990).

  16. 16.

    Nature 279, 643–645 (1979).

  17. 17.

    CRC crit. Rev. Biochem. 19, 145–190 (1985).

  18. 18.

    et al. Neuron 6, 305–320 (1991).

  19. 19.

    et al. Nature 353, 73–75 (1991).

  20. 20.

    & Molec. biochem. Parasit. 50, 161–177 (1992).

  21. 21.

    , , & Am. J. trop. Med. Hyg. 39, 157–165 (1988).

  22. 22.

    , , & Molec. biochem. Parasit. 21, 83–92 (1986).

  23. 23.

    , & Comp. biochem. Physiol. 91B, 735–739 (1988).

  24. 24.

    & Nature 355, 167–169 (1992).

  25. 25.

    et al. J. exp. Med. 173, 961–969 (1991).

  26. 26.

    , , & Neuron 6, 13–20 (1991).

  27. 27.

    & Science 193, 673–675 (1976).

  28. 28.

    & J. Parasit. 65, 418–420 (1979).

  29. 29.

    & J. gen. Physiol. 90, 453–478 (1987).

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Author information

Affiliations

  1. Department of Cell Biology, Washington University, St Louis, 660 S. Euclid Avenue, St Louis, Missouri 63110, USA

    • Sanjay A. Desai
    • , Donald J. Krogstad
    •  & Edwin W. McCleskey
  2. Department of Medicine, Pathology and Mechanical Engineering, Washington University, St Louis, 660 S. Euclid Avenue, St Louis, Missouri 63110, USA

    • Donald J. Krogstad
  3. Present addresses: Department of Medicine. Box 31215, Duke University Medical Center, Durham. NC 27710 (S.A.D.): Tulane School of Public Health and Tropical Medicine. 1501 Canal Street. New Orleans. LA 70112. USA (D.J.K.).

    • Sanjay A. Desai
    •  & Donald J. Krogstad

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https://doi.org/10.1038/362643a0

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