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Structure of a major immunogenic site on foot-and-mouth disease virus

Nature volume 362, pages 566568 (08 April 1993) | Download Citation

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Abstract

ATTACHMENT of foot-and-mouth disease virus (FMDV) to its cellular receptor involves a long and highly antigenic loop containing the conserved sequence, Arg-Gly-Asp, a motif known to be a recognition element in many integrin-dependent cell adhesion processes1–7. In our original crystal structure of FMDV the Arg-Gly-Asp-containing loop ('the loop'), located between β-strands G and H of capsid protein VP1, was disordered and hence essentially invisible. We previously surmised that its disorder is enhanced by a disulphide bond linking the base of the loop (Cys 134) to Cys 130 of VP2 (ref. 8). We report here the crystal structure of the virus in which this disulphide is reduced. Reduced virus retains infectivity and serological experiments suggest that some of the loop's internal structure is conserved8. But here its structure has become sufficiently ordered to allow us to describe an unambiguous conformation, which we relate to some key biological properties of the virus.

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References

  1. 1.

    , & J. gen. Virol. 59, 295–306 (1982).

  2. 2.

    et al. Nature 298, 30–33 (1982).

  3. 3.

    , , , & EMBO J. 1, 869–874 (1982).

  4. 4.

    , , , & Soviet J. Bioorg. Chem. 14, 572–580 (1989).

  5. 5.

    et al. in Vaccines 89: Modern Approaches to New Vaccines Including Prevention of AIDS (Cold Spring Harbor Laboratory Press, New York, 1989).

  6. 6.

    et al. J. gen. Virol. 70, 625–637 (1989).

  7. 7.

    & Virus Genes 4, 73–80 (1990).

  8. 8.

    et al. Nature 347, 569–572 (1990).

  9. 9.

    et al. J. molec. Biol. 196, 591–597 (1987).

  10. 10.

    et al. Nature 337, 709–716 (1989).

  11. 11.

    et al. Nature 308, 694–697 (1983).

  12. 12.

    et al. Eur. J. Biochem. 199, 545–551 (1991).

  13. 13.

    , & Proc. natn. Acad. Sci. U.S.A. 82, 178–182 (1985).

  14. 14.

    , , , & J. gen. Virol. 73, 621–626 (1992).

  15. 15.

    , , , & Science 255, 1718–1720 (1992).

  16. 16.

    & Nature 309, 30–33 (1984).

  17. 17.

    A. Rev. Biochem. 57, 375–413 (1988).

  18. 18.

    et al. J. molec. Biol. 112, 535–542 (1977).

  19. 19.

    et al. J. molec. Biol. 170, 175–202 (1983).

  20. 20.

    , , & J. molec. Biol. 184, 479–502 (1985).

  21. 21.

    & J. molec. Biol. 160, 623–639 (1982).

  22. 22.

    et al. J. molec. Biol. 210, 91–111 (1989).

  23. 23.

    et al. FEBS Lett. 291, 50–54 (1991).

  24. 24.

    et al. Eur. J. Biochem. 178, 141–154 (1988).

  25. 25.

    , & Biochemistry 30, 7369–7372 (1991).

  26. 26.

    , , & Science 235, 445–448 (1991).

  27. 27.

    , , & Science 258, 987–991 (1992).

  28. 28.

    , & Acta crystallogr. A49, 45–55 (1993).

  29. 29.

    , & Science 235, 458–460 (1987).

  30. 30.

    & Acta crystallogr. A34, 517–525 (1978).

  31. 31.

    , , & Acta crystallogr. A47, 110–119 (1991).

  32. 32.

    J. appl. Crystallogr. 24, 946–950 (1991).

  33. 33.

    et al. Nature 343, 767–770 (1990).

  34. 34.

    , , & Protein Eng. 1, 467–469 (1987).

  35. 35.

    & Biotechnology 8, 27–31 (1990).

  36. 36.

    , , , & Science 249, 1307–1309 (1990).

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Author information

Affiliations

  1. Laboratory of Molecular Biophysics, Rex Richards Building, University of Oxford, South Parks Road, Oxford 0X1 3QU, UK

    • Derek Logan
    • , Susan Lea
    • , Richard Lewis
    • , David Stuart
    •  & Elizabeth Fry
  2. AFRC Institute for Animal Health, Ash Road, Pirbright, Woking GU24 ONF, UK

    • Robin Abu-Ghazaleh
    • , Wendy Blakemore
    • , Stephen Curry
    • , Terry Jackson
    • , Andrew King
    •  & John Newman
  3. Department of Molecular Sciences, Wellcome Research Laboratories, Langley Court, Beckenham BR3 3BS, UK

    • Nigel Parry
    •  & David Rowlands
  4. Present address: UPR de Biologie Structurale, IBMC du CNRS, 15 rue René Descartes, 67084 Strasbourg Cedex, France

    • Derek Logan

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https://doi.org/10.1038/362566a0

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