Article | Published:

Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator

Nature volume 362, pages 511517 (08 April 1993) | Download Citation

Subjects

Abstract

The TFIID complex consists of the TATA-binding protein (TBP) and associated factors (TAFs) serving to mediate transcriptional activation by promoter-specific regulators. Here we report the cloning of Drosophila TAFII250 and the assembly of a partial complex containing recombinant TBP, TAFII110 and the C-terminal domain of TAFII250. This triple complex supports Sp1 activation and reveals specific interactions between TAFII250, TBP and TAFII110.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    & Curr. Opin. Genet. Dev. 2, 236–242 (1992).

  2. 2.

    & Curr. Opin. Cell Biol. 4, 488–495 (1992).

  3. 3.

    , , & Cell 54, 665–669 (1988).

  4. 4.

    & Cell 43, 165–175 (1985).

  5. 5.

    , & Nature 345, 783–786 (1990).

  6. 6.

    & Cell 61, 1187–1197 (1990).

  7. 7.

    , , & Science 248, 1625–1630 (1990).

  8. 8.

    , & Cell 55, 563–576 (1991).

  9. 9.

    et al. Cell 72, 247–270 (1993).

  10. 10.

    , & Genes Dev. 5, 2212–2224 (1991).

  11. 11.

    & J. biol. Chem. 267, 679–682 (1992).

  12. 12.

    , , & Genes Dev. 6, 1964–1974 (1992).

  13. 13.

    , & Cell 68, 965–976 (1992).

  14. 14.

    , & Cell 71, 1015–1028 (1992).

  15. 15.

    , , & Cell 71, 1029–1040 (1992).

  16. 16.

    & Cell 71, 1041–1054 (1992).

  17. 17.

    et al. Cell 71, 1055–1064 (1992).

  18. 18.

    Curr. Biol. 2, 565–567 (1992).

  19. 19.

    et al. Proc. natn. Acad Sci. U.S.A. 89, 11809–11813 (1992).

  20. 20.

    , & Nature 362, 175–179 (1993).

  21. 21.

    , & J. biol. Chem. 265, 21223–21231 (1990).

  22. 22.

    , , , & Molec. cell Biol. 11, 3317–3325 (1991).

  23. 23.

    et al. Nature 362, 179–181 (1993).

  24. 24.

    & Genes Dev. 5, 1935–1945 (1991).

  25. 25.

    , , , & Genes Dev. 6, 1319–1331 (1992).

  26. 26.

    , , & Cell 69, 883–894 (1992).

  27. 27.

    , & Genes Dev. 7, 180–187 (1993).

  28. 28.

    et al. Cell 70, 351–364 (1992).

  29. 29.

    , , , & Cell 61, 1179–1186 (1990).

  30. 30.

    , & Cell 53, 577–587 (1988).

  31. 31.

    et al. Proc. natn. Acad. Sci. U.S.A. 88, 1247–1250 (1991).

Download references

Author information

Author notes

    • Brian David Dynlacht

    Present address: Massachusetts General Hospital, Cancer Center, Charlestown, Massachusetts 02129, USA.

Affiliations

  1. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 401 Barker Hall, University of California at Berkeley, Berkeley, California 94720, USA

    • Robert O. J. Weinzierl
    • , Brian David Dynlacht
    •  & Robert Tjian

Authors

  1. Search for Robert O. J. Weinzierl in:

  2. Search for Brian David Dynlacht in:

  3. Search for Robert Tjian in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/362511a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.