Letter | Published:

TFIIIC relieves repression of U6 snRNA transcription by chromatin

Nature volume 362, pages 475477 (01 April 1993) | Download Citation

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Abstract

THE U6 small nuclear (sn)RNA gene (SNR6) from the yeast Saccharomyces cerevisiae is transcribed by RNA polymerase III in vivo1. This gene is unusual in having a TATA box at position –30, and an essential B-block element located downstream of the T-rich termination signal2, 3. The B block is one of the two intragenic promoter elements of transfer RNA genes that are recognized by transcription factor (TF)IIIC (ref. 4). But accurate in vitro transcription of yeast U6 snRNA gene by PolIII in a purified system requires only TFIIIB components, including the TATA-box binding protein TBP5. Here we report that, after nucleosome reconstitution or chromatin assembly, U6 snRNA synthesis becomes dependent on TFIIIC and on the integrity of the B-block element. This observation resolves an apparent paradox between in vitro and in vivo results concerning the necessity of the downstream B-block element3, 5 and sheds light on a new role of TFIIIC in gene activation.

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Affiliations

  1. Srvice de Biochimie et Génétique Moléculaire, Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes de Saclay, F 91191 Gif-sur-Yvette Cedex, France

    • Anne-Fran¸oise Burnol
    • , Florence Margottin
    • , Janine Huet
    •  & André Sentenac
  2. Laboratoire d'Embryologie Moleculaire, Institut Jacques Monod, 2 place Jussieu, F–75251 Paris Cedex, France

    • Geneviève Almouzni
    • , Marie-Noëlle Prioleau
    •  & Marcel Méchali
  3. Present address: National Institute of Health, NICHD, Bethesda, Maryland 20892, USA

    • Geneviève Almouzni

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https://doi.org/10.1038/362475a0

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