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Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis

Nature volume 362, pages 466468 (01 April 1993) | Download Citation

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Abstract

THE transcription factor GATA-1 is a fundamental regulator of genes in haematopoietic cell lineages1, 2 and belongs to a family of factors that bind to the consensus sequence WGATAR3–5. The GAT A motif was originally identified in cis-regulatory regions of globin and other erythroid-specific genes6, 7, but the range of genes controlled by the GATA factors has since expanded1, 7–10. Members of the GATA transcription factor family share a conserved zinc-finger DNA-binding domain, but the expression profile of each GATA factor is distinct11–13. Here we show that a testis form of murine (m)GATA-l messenger RNA is transcribed from a promoter located 5' to the erythroid first exon, and the remaining exons (which encode the mGATA-1 protein) are used in common by both testis and erythroid transcripts. We use an anti-mGATA-1 monoclonal antibody to show that the factor expressed in erythroid cells is the same as that found in the seminiferous tubules of murine testis. The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis

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Author information

Affiliations

  1. Department of Pediatrics, Hirosaki University School of Medicine, Hirosaki 036, Japan

    • Etsuro Ito
    • , Tsutomu Toki
    •  & Masaru Yokoyama
  2. Department of Biochemistry, Tohoku University School of Medicine, Seiryomachi, Aoba-ku, Sendai 980, Japan

    • Hajime Ishihara
    •  & Masayuki Yamamoto
  3. Department of Pathology, Tohoku University School of Medicine, Seiryomachi, Aoba-ku, Sendai 980, Japan

    • Haruo Ohtani
  4. Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA

    • Lin Gu
    •  & James Douglas Engel
  5. To whom correspondence should be addressed

    • Masayuki Yamamoto

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https://doi.org/10.1038/362466a0

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