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Regulation of apical transport in epithelial cells by a Gsclass of heterotrimeric G protein

Naturevolume 362pages456458 (1993) | Download Citation

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Abstract

THE role of heterotrimeric GTP-binding proteins in signal trans-duction is well established1. They might also be involved in vesicular transport2–6. Here we show that in the epithelial cell line Madin–Darby Canine Kidney, transport of influenza haemagglutinin protein to the apical surface is stimulated and that of vesicular stomatitis virus glycoprotein to the basolateral surface is retarded by A1F(3–5) treatment. Treatment of cells with the reagents known to influence the Gj class of G proteins affected only the basolateral pathway whereas reagents acting on the Gs class of G proteins specifically affected the apical pathway. In permeabilized cells, antibodies raised against the N-terminal domain of the α-subunit of Gs inhibited the transport of haemagglutinin from the trans-Golgi network to apical surface but not between the endoplasmic reticulum and Golgi complex. These observations demonstrate involvement of a Gs class of heterotrimeric G proteins, besides that of the Gi, in vesicular transport2. Moreover, the apical and the basolateral pathways in epithelial cells seem to be regulated by Gs and Gt proteins, respectively, in the trans- Golgi network.

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  1. Kai Simons: To whom correspondence should be addressed.

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  1. Cell Biology Programme, European Molecular Biology Laboratory, Postfach 102209, 6900, Heidelberg, Germany

    • Sanjay W. Pimplikar
    •  & Kai Simons

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https://doi.org/10.1038/362456a0

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