Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

lnterleukin-13 is a new human lymphokine regulating inflammatory and immune responses

Abstract

THE discovery of new cytokines normally relies on a prior knowledge of at least one of their biological effects, which is used as a criterion either for the purification of the protein or for the isolation of the complementary DNA by expression cloning. However, the redundancy of cytokine activities complicates the discovery of novel cytokines in this way, and the pleiotropic nature of many cytokines means that the principal activities of a new cytokine may bear little relation to that used for its isolation. We have adopted an alternative approach which relies on differential screening of an organized subtracted cDNA library from activated peripheral blood mononuclear cells, using the inducibility of lymphokine messenger RNAs by anti-CD28 as a primary screening criterion. The ligation of the CD28 antigen on the T lymphocyte by a surface antigen, B7/BB-1, expressed on activated B lymphocytes and monocytes1,2 is a key step in the activation of T lymphocytes and the accumulation of lymphokine mRNAs3. Here we report the discovery by molecular cloning of a new interleukin (interleukin-13 or IL-13) expressed in activated human T lymphocytes. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-2 in regulating interferon-γ synthesis in large granular lymphocytes. Recent mapping of the IL-13 gene shows that it is closely linked to the IL-4 gene on chromosome 5q 23–31 (ref. 4). Interleukin-13 may be critical in regulating inflammatory and immune responses.

Your institute does not have access to this article

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Linsley, P. S., Clark, E. A. & Ledbetter, J. A. Proc. natn. Acad. Sci. U.S.A. 87, 5031–5035 (1990).

    ADS  CAS  Article  Google Scholar 

  2. Gimmi, C. D. et al. Proc. natn. Acad. Sci. U.S.A. 88, 6575–6579 (1991).

    ADS  CAS  Article  Google Scholar 

  3. Thompson, C. B. et al. Proc. natn. Acad. Sci. U.S.A. 86, 1333–1337 (1989).

    ADS  CAS  Article  Google Scholar 

  4. Morgan, J. G., Dolganov, G. M., Robbins, S. E., Hinton, L. M. & Lovett, M. Nucleic Acids Res. 20, 5173–5179 (1992).

    CAS  Article  Google Scholar 

  5. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    CAS  Article  Google Scholar 

  6. von Heijne, G. Nucleic Acids Res. 14, 4683–4690 (1986).

    CAS  Article  Google Scholar 

  7. Caput, D. et al. Proc. natn. Acad. Sci. U.S.A. 83, 1670–1674 (1986).

    ADS  CAS  Article  Google Scholar 

  8. Shaw, G. & Kamen, R. Cell 46, 659–667 (1986).

    CAS  Article  Google Scholar 

  9. Powers, R. et al. Science 256, 1673–1677 (1992).

    ADS  CAS  Article  Google Scholar 

  10. Morrison, B. W. & Leder, P. J. biol. Chem. 267, 11957–11963 (1992).

    CAS  PubMed  Google Scholar 

  11. Brown, K. D., Zurawski, S. M., Mosmann, T. R. & Zurawski, G. J. Immun. 142, 679–687 (1989)

    CAS  PubMed  Google Scholar 

  12. Cherwinski, H. M., Schumacher, J. H., Brown, K. D. & Mosmann, T. R. J. exp. Med. 166, 1229–1244 (1987).

    CAS  Article  Google Scholar 

  13. de Waal Malefyt, R., Abrams, J., Bennett, B., Figdor, C. G. & de Vries J. E. J. exp. Med. 174, 1209–1220 (1991).

    CAS  Article  Google Scholar 

  14. Montaner, L. J. et al. J. Cell. Biochem. Abstr. suppl. 17B, 94 (1993).

    Google Scholar 

  15. Koyanagi, Y. et al. Science 241, 1673–1675 (1988).

    ADS  CAS  Article  Google Scholar 

  16. Scott, P. J. Immun. 147, 3149–3155 (1991).

    CAS  PubMed  Google Scholar 

  17. Kobayashi, M. et al. J. exp. Med. 170, 827–845 (1989).

    CAS  Article  Google Scholar 

  18. Boom, W. H., Liano, D. & Abbas, A. K. J. exp. Med. 167, 1350–1363 (1988).

    CAS  Article  Google Scholar 

  19. Zlotnik, A. & Moore, K. Cytokine 3, 366–371 (1991).

    CAS  Article  Google Scholar 

  20. Cher, D. J. & Mosmann, T. R. J. Immun. 138, 3688–3694 (1987).

    CAS  PubMed  Google Scholar 

  21. Ohlsson, K., Bjork, P., Bergenfeldt, M., Hageman, R. & Thompson, R. C. Nature 348, 550–552 (1990).

    ADS  CAS  Article  Google Scholar 

  22. Williams, R. O., Feldmann, M. & Maini, R. N. Proc. natn. Acad. Sci. U.S.A. 89, 9784–9788 (1992).

    ADS  CAS  Article  Google Scholar 

  23. Seed, B. & Aruffo, A. Proc. natn. Acad. Sci. U.S.A. 84, 3365–3369 (1987).

    ADS  CAS  Article  Google Scholar 

  24. Dorner, A. J., Bole, D. G. & Kaufman, R. J. J. cell. Biol. 105, 2665–2674 (1987).

    CAS  Article  Google Scholar 

  25. Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12, 387–395 (1984).

    CAS  Article  Google Scholar 

  26. Aarden, L. A., DeGroot, E. R., Schapp, O. L. & Lansdorp, P. M. Eur. J. Immun. 17, 1411–1416 (1987).

    CAS  Article  Google Scholar 

  27. Brake, A. J. Meth. Enzym. 185, 408–421 (1990).

    CAS  Article  Google Scholar 

  28. Timonen, T., Reynolds, C. W., Ortaldo, J. R. & Herberman, R. B. J. Immun. Meth. 51, 269–277 (1982).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Minty, A., Chalon, P., Derocq, JM. et al. lnterleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature 362, 248–250 (1993). https://doi.org/10.1038/362248a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/362248a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing