Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Product Review
  • Published:

Magnetic bead capture of expressed sequences encoded within large genomic segments

Nature volume 361, pages 751–753 (1993)Cite this article

Magnetic bead capture utilizes biotin–streptavidin magnetic bead technology to isolate cDNAs rapidly from large genomic intervals, giving several thousand-fold enrichment of the selected cDNAs. The technique can allow parallel analysis of several large genomic segments of varying complexities and can be applied to the isolation of expressed sequences from various tissue sources.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Collins, F. S. Nature Genet. 1, 3–6 (1992).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Liu, P., Legerski, R. & Siciliano, M. J. Science 246, 813–815 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Corbo, L., Maley, J. A., Nelson, D. L. & Caskey, C. T. Science 249, 652–655 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Duyk, G. M., Kim, S. W., Myers, R. M. & Cox, D. R. Proc. natn. Acad. Sci. U.S.A. 87, 8995–8999 (1990).

    Article  ADS  CAS  Google Scholar 

  5. Buckler, A. J. et al. Proc. natn. Acad. Sci. U.S.A. 88, 4005–4009 (1991).

    Article  ADS  CAS  Google Scholar 

  6. Wallace, M. R. et al. Science 249, 181–186 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Elvin, P. et al. Nucleic Acids Res. 18, 3913–3917 (1990).

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  8. Lovett, M., Kere, J. & Hinton, L. M. Proc. natn. Acad. Sci. U.S.A. 88, 9628–9632 (1991).

    Article  ADS  CAS  Google Scholar 

  9. Parimoo, S., Patanjali, S. R., Shukla, H., Chaplin, D. D. & Weissman, S. M. Proc. natn. Acad. Sci. U.S.A. 88, 9623–9627 (1991).

    Article  ADS  CAS  Google Scholar 

  10. Tagle, D. A. et al. Am. J. hum. Genet. 51, A22 (1992).

    Google Scholar 

  11. Morgan, J. G., Dolganov, G. M., Robbins, S. E., Hinton, L. M. & Lovett, M. Nucleic Acids Res. 20, 5173–5179 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Korn, B. et al. Hum. molec. Genet. 1, 235–242 (1992).

    Article  CAS  PubMed  Google Scholar 

  13. Bates, G. P. et al. Am. J. hum. Genet. 49, 7–16 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Bates, G. P. et al. Nature Genet. 1, 180–187 (1992).

    Article  CAS  PubMed  Google Scholar 

  15. Britten, R. J., Graham, D. E. & Neufeld, B. R. Meth. Enzym. 29, 363–418 (1974).

    Article  CAS  PubMed  Google Scholar 

  16. Britten, R. J. & Davidson, E. H. in Nucleic Acid Hybridization—A Practical Approach (eds Hames, B. D. & Higgins, S. J.) 3–15 (IRL, Oxford, 1985).

    Google Scholar 

  17. Young, B. D. & Anderson, M. L. M. in Nucleic Acid Hybridization—A Practical Approach (eds Hames, B. D. & Higgins, S. J.) 47–71 (IRL, Oxford, 1985).

    Google Scholar 

  18. Haukanes, B. -I. & Kvam, C. Bio/technology 11, 60–63 (1993).

    CAS  Google Scholar 

  19. Adams, M. D. et al. Science 355, 632–634 (1992).

    CAS  Google Scholar 

  20. Taylor, S. A. M. et al. Nature Genet. 2, 223–227 (1992).

    Article  CAS  PubMed  Google Scholar 

  21. Snell, R. G. et al. Hum. molec. Genet. (in the press).

Download references

Author information

Authors and Affiliations

  1. Departments of Human Genetics, University of Michigan Medical Center, Ann Arbor, Michigan, 48109–0650, USA

    Danilo A. Tagle & Francis S. Collins

  2. Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, 48109–0650, USA

    Francis S. Collins

  3. Howard Hughes Medical Institute,

    Manju Swaroop & Francis S. Collins

  4. Department of Biochemistry, University of Texas - Southwestern Medical Center, Dallas, Texas, 75235, USA

    Michael Lovett

Authors
  1. Danilo A. Tagle
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manju Swaroop
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael Lovett
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Francis S. Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tagle, D., Swaroop, M., Lovett, M. et al. Magnetic bead capture of expressed sequences encoded within large genomic segments. Nature 361, 751–753 (1993). https://doi.org/10.1038/361751a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/361751a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing