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Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination

Nature volume 360, pages 597–599 (1992)Cite this article

Abstract

ORNITHINE decarboxylase (ODC), a key enzyme in polyamine biosynthesis, is the most rapidly turned over mammalian enzyme1. We have shown that its degradation is accelerated by ODC antizyme2,3 an inhibitory protein induced by poly amines4,5. This is a new type of enzyme regulation and may be a model for selective protein degradation. Here we report the identification of the protease responsible for ODC degradation. Using a cell-free degradation system, we demonstrate that immunodepletion of proteasomes from cell extracts causes almost complete loss of ATP-and antizyme-dependent degradation of ODC. In addition, purified 26S proteasome complex, but not the 20S proteasome, catalyses ODC degradation in the absence of ubiquitin. These results strongly suggest that the 26S proteasome, widely viewed as specific for ubiquitin-conjugated proteins, is the main enzyme responsible for ODC degradation. The 26S proteasome may therefore have a second role in ubiquitin-independent proteolysis.

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Author notes

  1. Kazuei Igarashi: Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho, Chiba 260, Japan

  2. Tomohiro Tamura, Keiji Tanaka and Akira Ichihara: Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan

Affiliations

  1. Department of Nutrition, The Jikei University School of Medicine, Minato-ku, Tokyo, 105, Japan

    Yasuko Murakami, Senya Matsufuji, Takaaki Kameji, Shin-ichi Hayashi, Kazuei Igarashi, Tomohiro Tamura, Keiji Tanaka & Akira Ichihara

Authors
  1. Yasuko Murakami
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  2. Senya Matsufuji
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  3. Takaaki Kameji
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  4. Shin-ichi Hayashi
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  5. Kazuei Igarashi
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  6. Tomohiro Tamura
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  7. Keiji Tanaka
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  8. Akira Ichihara
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Murakami, Y., Matsufuji, S., Kameji, T. et al. Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination. Nature 360, 597–599 (1992). https://doi.org/10.1038/360597a0

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