Abstract
THE fungal metabolite brefeldin A is a powerful tool for investigating membrane traffic in eukaryotic cells1. The effects of brefeldin A on traffic are partly explained by its ability to prevent binding of cytosolic coat proteins onto membranes2–5. The non-clathrin coatomer complex6,7 binds reversibly to Golgi membranes in a GTP-controlled cycle8–10. The low-molecular-mass GTP-binding protein ADP-ribosylation factor (ARF), which also associates reversibly with Golgi membranes11,12, is required for coatomer binding13 and probably accounts for the control by guanine nucleotide of the coatomer–membrane interaction. Brefeldin A prevents the assembly of coatomer onto the membrane by inhibiting the GTP-dependent interaction of ARF with the Golgi membrane13, but the nature of this interaction has not been established. Here we demonstrate that Golgi membranes can specifically catalyse the exchange of GTP onto ARF and that brefeldin A prevents this function.
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References
Klausner, R. D., Donaldson, J. G. & Lippincott-Schwartz, J. J. Cell Biol. 116, 1071–1080 (1992).
Donaldson, J. G., Lippincott-Schwartz, J., Bloom, G. S., Kreis, T. E. & Klausner, R. D. J. Cell Biol. 111, 2295–2306 (1990).
Orci, L. et al. Cell 64, 1183–1195 (1991).
Robinson, M. S. & Kreis, T. E. Cell 69, 129–138 (1992).
Narula, N. et al. J. Cell Biol. 117, 27–38 (1992).
Serafini, T. et al. Nature 349, 215–220 (1991).
Waters, M. G., Serafini, T. & Rothman, J. E. Nature 349, 248–251 (1991).
Melançon, P. et al. Cell 51, 1053–1062 (1987).
Donaldson, J. G., Lippincott-Schwartz, J. & Klausner, R. D. J. Cell Biol. 112, 579–588 (1991).
Donaldson, J. G., Kahn, R. A., Lippincott-Schwartz, J. & Klausner, R. D. Science 254, 1197–1199 (1991).
Stearns, T., Willingham, M. C., Botstein, D. & Kahn, R. A. Proc. natn. Acad. Sci. U.S.A. 87, 1238–1242 (1990).
Serafini, T. et al. Cell 67, 239–254 (1991).
Donaldson, J. G., Cassel, D., Kahn, R. A. & Klausner, R. D. Proc. natn. Acad. Sci. U.S.A. 89, 6408–6412 (1992).
Weiss, O., Holden, J., Rulka, C. & Kahn, R. A. J. biol. Chem. 264, 21066–21072 (1989).
Duronio, R. J. et al. Proc. natn. Acad. Sci. U.S.A. 87, 1506–1510 (1990).
Kahn, R. A. & Gilman, A. G. J. biol. Chem. 261, 7906–7911 (1986).
Balch, W. E., Kahn, R. A. & Schwaninger, R. J. biol. Chem. 267, 13053–13061 (1992).
Kahn, R. A. et al. J. biol. Chem. 267, 13039–13046 (1992).
Lenhard, J. M., Kahn, R. A. & Stahl, P. D. J. biol. Chem. 267, 13047–13052 (1992).
Taylor, T. C., Kahn, R. A. & Melançon, P. Cell 70, 69079 (1992).
Bourne, H. R., Sanders, D. A. & McCormick, F. Nature 348, 125–131 (1990).
Hall, A. Science 249, 635–640 (1990).
Kikuchi, A. et al. J. biol. Chem. 267, 14611–14615 (1992).
Shou, C., Farnsworth, C. L., Neel, B. G. & Feig, L. A. Nature 358, 351–354 (1992).
Balch, W, E., Glick, B. S. & Rothman, J. E. Cell 39, 525–536 (1984).
Kahn, R. A. Meth. Enzym. 195, 233–243 (1991).
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Donaldson, J., Finazzi, D. & Klausner, R. Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein. Nature 360, 350–352 (1992). https://doi.org/10.1038/360350a0
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DOI: https://doi.org/10.1038/360350a0
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