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CENP-E is a putative kinetochore motor that accumulates just before mitosis

Nature volume 359, pages 536–539 (1992)Cite this article

Abstract

THE mechanics of chromosome movement, mitotic spindle assembly and spindle elongation have long been central questions of cell biology1. After attachment in prometaphase of a micro-tubule from one pole, duplicated chromosome pairs travel towards the pole in a rapid but discontinuous motion2,3. This is followed by a slower congression towards the midplate as the chromosome pair orients with each kinetochore attached to the microtubules from the nearest pole. The pairs disjoin at anaphase and translocate to opposite poles and the interpolar distance increases. Here we identify CENP-E as a kinesin-like motor protein (Mr 312,000) that accumulates in the G2 phase of the cell cycle. CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. CENP-E is likely to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.

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Authors and Affiliations

  1. Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania, 19111, USA

    Tim J. Yen & Gang Li

  2. Cell Biology Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA

    Bruce T. Schaar

  3. Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland, 21205, USA

    Illya Szilak & Don W. Cleveland

Authors
  1. Tim J. Yen
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  2. Gang Li
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  3. Bruce T. Schaar
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  4. Illya Szilak
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  5. Don W. Cleveland
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Yen, T., Li, G., Schaar, B. et al. CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature 359, 536–539 (1992). https://doi.org/10.1038/359536a0

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