Abstract
A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.
Similar content being viewed by others
Article PDF
References
Montanaro, V. et al. Am. J. hum. Genet. 48, 183–194 (1991).
Korenberg, J. R. & Tykowski, M. C. Cell 53, 391–400 (1988).
Cox, D. R. et al. Science 250, 245–250 (1990).
Gardiner, K. et al. Somat. Cell molec. Genet. 14, 623–638 (1988).
Coulson, A., Sulston, J., Brenner, S. & Kam, J. Proc. natn. Acad. Sci. U.S.A. 83, 7821–7825 (1986).
Olson, M. et al. Proc. natn. Acad. Sci. U.S.A. 83, 7826–7830 (1986).
Evans, G. A. & Lewis, K. A. Proc. natn. Acad. Sci. U.S.A. 86, 5030–5034 (1989).
Craig, G. et al. Nucleic Acids Res. 18, 2653–2660 (1990).
Stallings, R. L. et al. Proc. natn. Acad. Sci. U.S.A. 87, 6218–6222 (1990).
Schlessinger, D. et al. Genomics 11, 783–793 (1991).
Botstein, D. et al. Am. J. hum. Genet. 32, 314–331 (1980).
Cox, D. R. et al. Cytogenet. Cell Genet. 58, 800–826 (1991).
Green, E. D. & Green, P. PCR Meth. Appl. 1, 77–90 (1991).
Burke, D. T., Carle, G. F. & Olson, M. V. Science 236, 806–812 (1987).
Anand, R., Villasente, A. & Tyler-Smith, C. Nucleic Acids Res. 17, 4325–4333 (1989).
Larin, Z., Monaco, A. P. & Lehrach, H. Proc. natn. Acad. Sci. U.S.A. 88, 4123–4127 (1991).
Albertsen, H. M. et al. Proc. natn. Acad. Sci. U.S.A. 87, 4256–4260 (1990).
Chumakov, I. M. et al. Nature Genet. 1, 222–225 (1992).
Olson, M. V. et al. Science 245, 1434–1435 (1989).
Ichikawa, H. et al. Proc. natn. Acad. Sci. U.S.A. 89, 23–27 (1992).
Tanzi, R. E. et al. Genomics (in the press).
Van Keuren, M. et al. Am. J. med. Genet. 38, 793–804 (1989).
Gardiner, K. et al. EMBO J. 9, 25–34 (1990).
Riethman, H. et al. Proc. natn. Acad. Sci. U.S.A. 86, 991–995 (1989).
Rigault, P. Int. J. Genome Res. (in the press).
Warburton, P. E. et al. Genomics 11, 324–333 (1991).
Assum, G. et al. Genomics 11, 397–404 (1991).
Petersen, M. B. et al. Genomics 9, 407–419 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chumakov, I., Rigault, P., Guillou, S. et al. Continuum of overlapping clones spanning the entire human chromosome 21q. Nature 359, 380–387 (1992). https://doi.org/10.1038/359380a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/359380a0
This article is cited by
-
Non-profit Drug Research and Development at a Crossroads
Pharmaceutical Research (2018)
-
The Bermuda Triangle: The Pragmatics, Policies, and Principles for Data Sharing in the History of the Human Genome Project
Journal of the History of Biology (2018)
-
Down syndrome and genetics — a case of linked histories
Nature Reviews Genetics (2005)
-
Functional Analysis of Genes Implicated in Down Syndrome: 1. Cognitive Abilities in Mice Transpolygenic for Down Syndrome Chromosomal Region-1 (DCR-1)
Behavior Genetics (2004)
-
Assembling a physical map of the genome by marker sequences
Journal of Forestry Research (2000)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
