Subjects

Abstract

A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.

References

  1. 1.

    et al. Am. J. hum. Genet. 48, 183–194 (1991).

  2. 2.

    & Cell 53, 391–400 (1988).

  3. 3.

    et al. Science 250, 245–250 (1990).

  4. 4.

    et al. Somat. Cell molec. Genet. 14, 623–638 (1988).

  5. 5.

    , , & Proc. natn. Acad. Sci. U.S.A. 83, 7821–7825 (1986).

  6. 6.

    et al. Proc. natn. Acad. Sci. U.S.A. 83, 7826–7830 (1986).

  7. 7.

    & Proc. natn. Acad. Sci. U.S.A. 86, 5030–5034 (1989).

  8. 8.

    et al. Nucleic Acids Res. 18, 2653–2660 (1990).

  9. 9.

    et al. Proc. natn. Acad. Sci. U.S.A. 87, 6218–6222 (1990).

  10. 10.

    et al. Genomics 11, 783–793 (1991).

  11. 11.

    et al. Am. J. hum. Genet. 32, 314–331 (1980).

  12. 12.

    et al. Cytogenet. Cell Genet. 58, 800–826 (1991).

  13. 13.

    & PCR Meth. Appl. 1, 77–90 (1991).

  14. 14.

    , & Science 236, 806–812 (1987).

  15. 15.

    , & Nucleic Acids Res. 17, 4325–4333 (1989).

  16. 16.

    , & Proc. natn. Acad. Sci. U.S.A. 88, 4123–4127 (1991).

  17. 17.

    et al. Proc. natn. Acad. Sci. U.S.A. 87, 4256–4260 (1990).

  18. 18.

    et al. Nature Genet. 1, 222–225 (1992).

  19. 19.

    et al. Science 245, 1434–1435 (1989).

  20. 20.

    et al. Proc. natn. Acad. Sci. U.S.A. 89, 23–27 (1992).

  21. 21.

    et al. Genomics (in the press).

  22. 22.

    et al. Am. J. med. Genet. 38, 793–804 (1989).

  23. 23.

    et al. EMBO J. 9, 25–34 (1990).

  24. 24.

    et al. Proc. natn. Acad. Sci. U.S.A. 86, 991–995 (1989).

  25. 25.

    Int. J. Genome Res. (in the press).

  26. 26.

    et al. Genomics 11, 324–333 (1991).

  27. 27.

    et al. Genomics 11, 397–404 (1991).

  28. 28.

    et al. Genomics 9, 407–419 (1991).

Download references

Author information

Affiliations

  1. Centre d'Etude du Polymorphisme Humain (CEPH), 27 rue Juliette Dodu, 75010 Paris, France

    • Ilya Chumakov
    • , Pierre Ougen
    • , Alain Billaut
    • , Isabelle LeGall
    • , Pascal Soularue
    • , Lydie Bougueleret
    • , Christine Bellanné-Chantelot
    • , Bruno Lacroix
    • , Guy Vaysseix
    • , Jean-Luc Sambucy
    • , Denis Le Paslier
    •  & Daniel Cohen
  2. Genethon, 1 rue de I'lnternationale, BP 59, 91002 Evry Cedex, France

    • Ilya Chumakov
    • , Philippe Rigault
    • , Sophie Guillou
    • , Ghislaine Guasconi
    • , Patricia Gervy
    • , Laurent Grinas
    • , Emmanuel Barillot
    • , Philippe Gesnouin
    • , Stuart Pook
    • , Guy Vaysseix
    • , Jean Weissenbach
    • , Alain Vignal
    •  & Daniel Cohen
  3. Centre d'Energie Atomique, 68 Avenue Division LeClerc, 92260 Fontenay aux Roses, France

    • Gerard Frelat
    •  & Annette Schmitz
  4. Institut de Recerca Oncologica, Hospital Duran i Reynals Ctra Castelldefels, KM 2.7, Barcelona 08907, Spain

    • Assumpcio Bosch
    •  & Xavier Estivill
  5. CNRS URA 1445, Institut Pasteur, 28 rue Docteur Roux, 75724 Paris, France

    • Jean Weissenbach
  6. The Wistar Institute, 36th Street and Spruce, Philadelphia, Pennsylvania 19104, USA

    • Harold Riethman
  7. Neurogenetics Laboratory, University of California, 401 Parnassus Avenue, San Francisco, California 94143, USA

    • David Cox
  8. Eleanor Roosevelt Institute, 1899 Gaylord Street, Denver, Colorado 80206, USA

    • David Patterson
    •  & Kathleen Gardiner
  9. The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minatoku, Tokyo 108, Japan

    • Masahira Hattori
    •  & Yoshiyuki Sakaki
  10. Saitama Cancer Center Research Institute, Ina-machi, Kitaadachi-gun, Saitama-ken 362, Japan

    • Hitoshi Ichikawa
    •  & Misao Ohki
  11. INSERM U184, Laboratoire de Génétique Moléculaire de Eucaryotes, 11 rue Humann, Strasbourg, France

    • Roland Heilig
  12. Center for Medical Genetics, The Johns Hopkins University School of Medicine, 600 North Wolfe Street CMSC 1003, Baltimore, Maryland 21205, USA

    • Stylianos Antonarakis

Authors

  1. Search for Ilya Chumakov in:

  2. Search for Philippe Rigault in:

  3. Search for Sophie Guillou in:

  4. Search for Pierre Ougen in:

  5. Search for Alain Billaut in:

  6. Search for Ghislaine Guasconi in:

  7. Search for Patricia Gervy in:

  8. Search for Isabelle LeGall in:

  9. Search for Pascal Soularue in:

  10. Search for Laurent Grinas in:

  11. Search for Lydie Bougueleret in:

  12. Search for Christine Bellanné-Chantelot in:

  13. Search for Bruno Lacroix in:

  14. Search for Emmanuel Barillot in:

  15. Search for Philippe Gesnouin in:

  16. Search for Stuart Pook in:

  17. Search for Guy Vaysseix in:

  18. Search for Gerard Frelat in:

  19. Search for Annette Schmitz in:

  20. Search for Jean-Luc Sambucy in:

  21. Search for Assumpcio Bosch in:

  22. Search for Xavier Estivill in:

  23. Search for Jean Weissenbach in:

  24. Search for Alain Vignal in:

  25. Search for Harold Riethman in:

  26. Search for David Cox in:

  27. Search for David Patterson in:

  28. Search for Kathleen Gardiner in:

  29. Search for Masahira Hattori in:

  30. Search for Yoshiyuki Sakaki in:

  31. Search for Hitoshi Ichikawa in:

  32. Search for Misao Ohki in:

  33. Search for Denis Le Paslier in:

  34. Search for Roland Heilig in:

  35. Search for Stylianos Antonarakis in:

  36. Search for Daniel Cohen in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/359380a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.