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Crystal structures explain functional properties of two E. coli porins

Nature volume 358, pages 727–733 (1992)Cite this article

Abstract

Porins form aqueous channels that aid the diffusion of small hydrophilic molecules across the outer membrane of Gram-negative bacteria. The crystal structures of matrix porin and phosphoporin both reveal trimers of identical subunits, each subunit consisting of a 16-stranded anti-parallel β-barrel containing a pore. A long loop inside the barrel contributes to a constriction of the channel where the charge distribution affects ion selectivity. The structures explain at the molecular level functional characteristics and their alterations by known mutations.

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References

  1. Jap, B. K. & Walian, P. J. Q. Rev. Biophys. 23, 367–403 (1990).

    CAS  Article  Google Scholar 

  2. Schirmer, T. & Rosenbusch, J. P. Curr. Opinion Struct. Biol. 1, 539–545 (1991).

    CAS  Article  Google Scholar 

  3. Nikaido, H. Molec. Microbiol. 6, 435–442 (1992).

    CAS  Article  Google Scholar 

  4. Mizuno, T., Chou, M.-Y. & Inouye, M. J. biol. Chem. 258, 6932–6940 (1983).

    CAS  PubMed  Google Scholar 

  5. Benz, R., Schmid, A. & Hancock, R. E. W. J. Bact. 162, 722–727 (1985).

    CAS  PubMed  Google Scholar 

  6. Dargent, B., Hofmann, W., Pattus, F. & Rosenbusch, J. P. EMBO J. 5, 773–778 (1986).

    CAS  Article  Google Scholar 

  7. Bühler, L. K., Kusumoto, S., Zhang, H. & Rosenbusch, J. P. J. biol. Chem. 266, 24446–24450 (1991).

    Google Scholar 

  8. Schindler, H. & Rosenbusch, J. P. Proc. natn. Acad Sci USA 75, 3751–3755 (1978).

    ADS  CAS  Article  Google Scholar 

  9. Henderson, R. & Unwin, P. N. T. Nature 257, 28–32 (1975).

    ADS  CAS  Article  Google Scholar 

  10. Deisenhofer, J., Epp, O., Miki, K., Huber, R. & Michel, H. Nature 318, 618–624 (1985).

    ADS  CAS  Article  Google Scholar 

  11. Rosenbusch, J. P. J. biol. Chem. 249, 8019–8029 (1974).

    CAS  Google Scholar 

  12. Garavito, R. M. & Rosenbusch, J. P. J. Cell Biol. 86, 327–329 (1980).

    CAS  Article  Google Scholar 

  13. Garavito, R. M., Jenkins, J., Jansonius, J. N., Karlsson, R. & Rosenbusch, J. P. J. molec. Biol. 164, 313–327 (1983).

    CAS  Article  Google Scholar 

  14. Weiss, M. S., Wacker, T., Weckesser, J., Welte, W. & Schulz, G. E. FEBS Lett 267, 268–272 (1990).

    CAS  Article  Google Scholar 

  15. Pauptit, R. A. et al. J. struct. Biol. 107, 136–145 (1991).

    CAS  Article  Google Scholar 

  16. Rossmann, M. G. The Molecular Replacement Method (Gordon & Breach, New York, 1972).

    Google Scholar 

  17. Richardson, J. Adv. Protein Chem. 34, 167–330 (1981).

    CAS  Article  Google Scholar 

  18. Lesk, A. M., Brändén, C.-I. & Chothia, C. Proteins Struct. Funct. Genet. 5, 139–148 (1989).

    CAS  Article  Google Scholar 

  19. Struyvé, M., Moons, M. & Tommassen, J. J. molec. Biol. 218, 141–148 (1991).

    Article  Google Scholar 

  20. Bosch, D., Scholten, M., Verhagen, C. & Tommassen, J. Molec Gen. Genet. 216, 144–148 (1989).

    CAS  Article  Google Scholar 

  21. Rosenbusch, J. P. & Müller, R. in Solubilization of Lipoprotein Complexes (eds Peeters, H. & Massue, J. P.) 59–68 (European, Ghent, 1977).

    Google Scholar 

  22. Jap, B. K., Downing, K. H. & Walian, P. J. J. struct. Biol. 103, 57–63 (1990).

    CAS  Article  Google Scholar 

  23. Sass, H. J. et al. J. molec. Biol. 209, 171–175 (1989).

    CAS  Article  Google Scholar 

  24. Jap, B. K. J. molec. Biol. 205, 407–419 (1989).

    CAS  Article  Google Scholar 

  25. Hönger, A., Gross, H., Aebi, U. & Engel, A. J. struct. Biol. 103, 185–195 (1990).

    Article  Google Scholar 

  26. Jap, B. K., Walian, P. J. & Gehring, K. Nature 350, 167–170 (1991).

    ADS  CAS  Article  Google Scholar 

  27. Engel, A., Massalski, A., Schindler, H., Dorset, D. L. & Rosenbusch, J. P. Nature 317, 643–645 (1985).

    ADS  CAS  Article  Google Scholar 

  28. Weiss, M. S. et al. FEBS Lett 280, 379–382 (1991).

    CAS  Article  Google Scholar 

  29. Lewis, B. A. & Engelman, D. M. J. molec. Biol. 166, 211–217 (1983).

    CAS  Article  Google Scholar 

  30. Weiss, M. S. et al. Science 254, 1627–1630 (1991).

    ADS  CAS  Article  Google Scholar 

  31. Deisenhofer, J. & Michel, H. Science 245, 1463–1473 (1989).

    ADS  CAS  Article  Google Scholar 

  32. Henderson, R. et al. J. molec Biol. 213, 899–929 (1990).

    CAS  Article  Google Scholar 

  33. Spurlino, J. C., Lu, G.-Y. & Quiocho, F. A. J. biol. Chem. 266, 5202–5219 (1991).

    CAS  PubMed  Google Scholar 

  34. Klebba, P. E. et al. J. biol. Chem. 265, 6800–6810 (1990).

    CAS  PubMed  Google Scholar 

  35. Steven, A. C., ten Heggeler, B., Müller, R., Kistler, J. & Rosenbusch, J. P. J. Cell Biol. 72, 292–301 (1977).

    CAS  Article  Google Scholar 

  36. Michel, H. Trends biochem. Sci. 8, 56–59 (1983).

    CAS  Article  Google Scholar 

  37. Benson, S. A., Occi, J. L. L. & Sampson, B. A. J. molec. Biol. 203, 961–970 (1988).

    CAS  Article  Google Scholar 

  38. Rocque, W. J. & McGroarty, E. J. Biochemistry 29, 5344–5351 (1990).

    CAS  Article  Google Scholar 

  39. Vogel, H. & Jähnig, F. J. molec. Biol. 190, 191–199 (1986).

    CAS  Article  Google Scholar 

  40. Hancock, R. E. W. J. Bact. 169, 929–933 (1987).

    CAS  Article  Google Scholar 

  41. Nikaido, H. & Rosenberg, E. Y. J. gen. Physiol. 77, 121–135 (1981).

    CAS  Article  Google Scholar 

  42. Nikaido, H. & Rosenberg, E. Y. J. Bact. 153, 241–252 (1983).

    CAS  PubMed  Google Scholar 

  43. Rosenbusch, J. P. Experientia 46, 167–173 (1990).

    CAS  PubMed  Google Scholar 

  44. Jeanteur, D., Lakey, J. H. & Pattus, F. Molec. Microbiol. 5, 2153–2164 (1991).

    CAS  Article  Google Scholar 

  45. Misra, R. & Benson, S. A. J. Bact. 170, 3611–3617 (1988).

    CAS  Article  Google Scholar 

  46. Lakey, J. H., Lea, E. J. A. & Pattus, F. FEBS Lett 278, 31–34 (1991).

    CAS  Article  Google Scholar 

  47. Bauer, K., Struyvé, M., Bosch, D., Benz, R. & Tommassen, J. J. biol. Chem. 264, 16393–16398 (1989).

    CAS  PubMed  Google Scholar 

  48. Messerschmidt, A. & Pflugrath, J. W. J. appl. Crystallogr. 20, 306–315 (1987).

    CAS  Article  Google Scholar 

  49. Pauptit, R. A. et al. J. molec. Biol. 218, 505–507 (1991).

    CAS  Article  Google Scholar 

  50. Steiert, M., Ghosh, R., Schirmer, T. & Rosenbusch, J. P. Experientia 48, A22 (1992).

    Article  Google Scholar 

  51. CCP4, SERC UK Collaborative Computing Project no. 4 (Daresbury Laboratory, Warrington, UK, 1979).

  52. Wang, B.-C. Meth. Enzym. 115, 90–112 (1985).

    CAS  Article  Google Scholar 

  53. Jones, T. A., Zou, J.-Y., Cowan, S. W. & Kjeldgaard, M. Acta Crystallogr. A 47, 110–119 (1991).

    Article  Google Scholar 

  54. Brünger, A. T. X-PLOR Manual (Yale Univ., New Haven, CO, 1990).

    Google Scholar 

  55. Jones, T. A. & Kjeldgaard, M. Manual for O (Uppsala Univ., Uppsala, Sweden, 1990).

    Google Scholar 

  56. Van der Ley, P., Struyvé, M. & Tommassen, J. J. biol. Chem. 261, 12222–12225 (1986).

    CAS  PubMed  Google Scholar 

  57. Korteland, J., Overbeeke, N., De Graaff, P., Overduin, P. & Lugtenberg, B. Eur. J. Biochem. 152, 691–697 (1985).

    CAS  Article  Google Scholar 

  58. Priestle, J. J. appl. Crystallogr. 21, 572–576 (1988).

    Article  Google Scholar 

Download references

Author information

Author notes

  1. R. A. Pauptit

    Present address: ICI Pharmaceuticals, Mereside, Alderley Park, Macclesfield, Cheshire, SK10 4TG, UK

Affiliations

  1. Department of Structural Biology, Biocentre, University of Basel, CH-4056, Basel, Switzerland

    S. W. Cowan, T. Schirmer, G. Rummel, M. Steiert, R. Ghosh, R. A. Pauptit, J. N. Jansonius & J. P. Rosenbusch

  2. Department of Microbiology, Biocentre, University of Basel, CH-4056, Basel, Switzerland

    G. Rummel, M. Steiert, R. Ghosh & J. P. Rosenbusch

Authors
  1. S. W. Cowan
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  2. T. Schirmer
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  3. G. Rummel
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  4. M. Steiert
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  5. R. Ghosh
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  6. R. A. Pauptit
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  7. J. N. Jansonius
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  8. J. P. Rosenbusch
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Cowan, S., Schirmer, T., Rummel, G. et al. Crystal structures explain functional properties of two E. coli porins. Nature 358, 727–733 (1992). https://doi.org/10.1038/358727a0

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  • DOI: https://doi.org/10.1038/358727a0

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