Molecular diversity of the NMDA receptor channel

Nature volume 358, pages36–41(1992)Cite this article

Abstract

Two novel subunits of the mouse NMDA receptor channel, the ɛ2 and ɛ3 subunits, have been identified by cloning and expression of complementary DMAs. The heteromeric ɛ1/ζ1, ɛ2/ζ1 and ɛ3/ζ1 NMDA receptor channels exhibit distinct functional properties in affinities for agonists and sensitivities to competitive antagonists and Mg2+ block. In contrast to the wide distribution of the ɛ1 and ζ1 subunit messenger RNAs in the brain, the ɛ2 subunit mRNA is expressed only in the forebrain and the ɛ3 subunit mRNA is found predominantly in the cerebellum. The ɛl/ζ1 and ɛ2/ζ1 channels expressed in Xenopus oocytes, but not the ɛ3/ζ1 channel, are activated by treatment with 12-O-tetradecanoylphorbol 13-acetate. These findings suggest that the molecular diversity of the ɛ subunit family underlies the functional heterogeneity of the NMDA receptor channel.

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Affiliations

  1. Department of Neuropharmacology, School of Medicine, Niigata University, Niigata, 951, Japan

    Tatsuya Kutsuwada, Nobuko Kashiwabuchi, Hisashi Mori, Kenji Sakimura, Etsuko Kushiya, Kazuaki Araki, Hiroyuki Meguro, Hisashi Masaki & Masayoshi Mishina

  2. Department of Neuropathology, Brain Research Institute, School of Medicine, Niigata University, Niigata, 951, Japan

    Toshiro Kumanishi

  3. Department of Medicine II, School of Medicine, Niigata University, Niigata, 951, Japan

    Tatsuya Kutsuwada, Hiroyuki Meguro & Masaaki Arakawa

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  1. Tatsuya Kutsuwada
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  2. Nobuko Kashiwabuchi
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  3. Hisashi Mori
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  6. Kazuaki Araki
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  7. Hiroyuki Meguro
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  8. Hisashi Masaki
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  11. Masayoshi Mishina
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Kutsuwada, T., Kashiwabuchi, N., Mori, H. et al. Molecular diversity of the NMDA receptor channel. Nature 358, 36–41 (1992). https://doi.org/10.1038/358036a0

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