Abstract
ANTIDIURESIS, the recovery of water from the lumen of the renal collecting tubule, is regulated by the hypothalamic release of antidiuretic hormone (ADH), which binds to specific receptors on renal collecting tubule cells, stimulates adenylyl cyclase and promotes the cyclic AMP-mediated incorporation of water pores into the luminal surface of these cells'1–3. We report here the isolation of the human ADH receptor gene using a genomic expression cloning approach4. The gene was used to clone the complementary DNA from a human renal library. The deduced amino-acid sequence of the receptor yields a hydropathy profile characteristic of receptors with seven putative transmembrane regions. This and the comparison with other cloned receptors indicates that the ADH receptor is a member of the superfamily of G-protein-coupled receptors.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Orloff, J. & Handler, J. S. Am. J. Med 42, 757–768 (1967).
Jard, S. in Current Topics in Membranes and Transport (Vol. 18, (ed. Kleinzeller, A.) 255–285 (Academic, New York, 1983).
Harris, H. W., Handler, J. S. & Blumenthal, R. Am. J. Physiol. 258, 237–243 (1990).
Birnbaumer, M., Hinrichs, V. & Themmen, A. P. N. Molec. Endocrin. 4, 245–254 (1990).
Wahl, G. M. Proc. natn. Acad. Sci. U.S.A. 84, 2160–2164 (1987).
Kaiser, K. & Murray, N. E. in DNA Cloning Vol. I (ed. Glober, D. M.) 1–48 (IRL, Oxford, England, 1985).
Birnbaumer, L., Nakahara, T. & Yang, P.-Ch. J. biol. Chem. 249, 7857–7866 (1974).
Michell, R. H., Kirk, C. J. & Billah, M. M. Biochem. Soc. Trans. 7, 861–865 (1979).
Jard, S., Lombard, C., Marie, J. & Devilliers, G. Am. J. Physiol. 253, 41–49 (1987).
Takeshima, H. et al. Nature 339, 439–445 (1989).
Lawrence, C. B., Shalom, T. Y. & Honda, S. Molec. biol. Inform. Resource (Baylor College of Medicine, Houston, Texas 77030, 1989).
Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).
Masu, Y. et al. Nature 329, 836–838 (1987).
Levy, F. O. et al. J. biol. Chem. (in the press).
Frielle, T. et al. Proc. natn. Acad. Sci. U.S.A. 84, 7920–7924 (1987).
Gudermann, T., Birnbaumer, M. & Birnbaumer, L. J. biol. Chem. 267, 4479–4488 (1992).
Liao, C.-F. et al. J. biol. Chem. 264, 7328–7337 (1989).
Lichter, P., Ledbetter, S. A., Ledbetter, D. H. & Ward, D. C. Proc. natn. Acad. Sci. U.S.A. 87, 6634–6638 (1990).
Kozack, M. J. Cell biol. 108, 229–241 (1989).
Woodget, J. R., Gould, K. L. & Hunter, T. Eur. J. Biochem. 161, 177–184 (1986).
Kennelly, P. J. & Krebs, E. G. J. biol. Chem. 266, 1555–1558 (1991).
Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).
Luthman, H. & Magnusson, G. Nucleic Acids Res. 11, 1295–1308 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Birnbaumer, M., Seibold, A., Gilbert, S. et al. Molecular cloning of the receptor for human antidiuretic hormone. Nature 357, 333–335 (1992). https://doi.org/10.1038/357333a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/357333a0
This article is cited by
-
Novel AVPR2 mutations and clinical characteristics in 28 Chinese families with congenital nephrogenic diabetes insipidus
Journal of Endocrinological Investigation (2021)
-
Focus on neonatal and infantile onset of nephrogenic syndrome of inappropriate antidiuresis: 12 years later
Pediatric Nephrology (2019)
-
Electron Microscopic Study of the Inner Medulla in Rat Kidneys under Conditions of Vasopressin Treatment Combined with Prostaglandin Synthesis Blockade
Bulletin of Experimental Biology and Medicine (2016)
-
Novel mutations associated with nephrogenic diabetes insipidus. A clinical-genetic study
European Journal of Pediatrics (2015)
-
Dynamic regulation and dysregulation of the water channel aquaporin-2: a common cause of and promising therapeutic target for water balance disorders
Clinical and Experimental Nephrology (2014)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
