Abstract
A multiprotein complex that specifically recognizes cellular origins of DNA replication has been identified and purified from the yeast Saccharomyces cerevisiae. We observe a strong correlation between origin function and origin recognition by this activity. Interestingly, specific DNA binding by the origin recognition complex is dependent upon the addition of ATP. We propose that the origin recognition complex acts as the initiator protein for S. cerevisiae origins of DNA replication.
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References
Borowiec, J. A., Dean, F. B., Bullock, P. A. & Hurwitz, J. Cell 60, 181–184 (1990).
Kornberg, A. & Baker, T. A. DNA Replication 2nd edn 1–849 (Freeman, New York, 1992).
Stillman, B. A. Rev. Cell Biol. 5, 197–245 (1989).
Challberg, M. D. & Kelly, T. J. A. Rev. Biochem. 58, 671–717 (1989).
Stinchcomb, D. T. Struhl, K. & Davis, R. W. Nature 282, 39–43 (1979).
Huberman, J. A., Spotilla, L. D., Nawotka, K. A., El-Assouli, S. M. & Davis, L. R. Cell 51, 473–481 (1987).
Brewer, B. J. & Fangman, W. L. Cell 51, 463–471 (1987).
Huberman, J. A., Zhu, J., Davis, L. R. & Newlon, C. S. Nucleic Acids Res. 16, 6373–6384 (1988).
Dubey, D. D. et al. Molec. cell. Biol. 11, 5346–5355 (1991).
Broach, J. et al. Cold Spring Harbor Symp. quant. Biol. 47, 1165–1173 (1983).
Newlon, C. S. Microbiol. Rev. 52, 568–601 (1988).
Marahrens, Y. & Stillman, B. Science 255, 817–823 (1992).
Buchman, A. R., Kimmerly, W. J., Rine, J. & Kornberg, R. Molec. cell. Biol. 8, 210–225 (1988).
Shore, D., Stillman, D. J., Brand, A. H. & Nasmyth, K. A. EMBO J. 6, 461–467 (1987).
Sweder, K. S., Rhode, P. R. & Campbell, J. L. J. biol. Chem. 263, 17270–17277 (1988).
Diffley, J. F. X. & Stillman, B. Proc. natn. Acad. Sci. U.S.A. 85, 2120–2124 (1988).
Jazwinski, S. M. & Edelman, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 3428–3432 (1982).
Jazwinski, S. M. & Edelman, G. M. J. biol. Chem. 259, 6852–6857 (1984).
Hofmann, J. F.-X. & Gasser, S. M. Cell 64, 951–960 (1991).
Schmidt, A. M. A., Herterich, S. U. & Krauss, G. EMBO J. 10, 981–985 (1991).
Kuno, K., Murakami, S. & Kuno, S. Gene 95, 73–77 (1990).
Travers, A. A. & Klug, A. Phil. Trans. R. Soc. B 317, 537–561 (1987).
Walker, S. C., Malik, A. K. & Eisenberg, S. Nucleic Acids Res. 19, 6255–6262 (1991).
Bouton, A. H. & Smith, M. M. Molec. cell. Biol. 6, 2354–2363 (1986).
Palzkill, T. G. & Newlon, C. S. Cell 37, 299–307 (1988).
Walker, S. C., Francesconi, S. C. & Eisenberg, S. Proc. natn. Acad. Sci. U.S.A. 87, 4665–4669 (1990).
Bartholomew, B., Kassavetis, G. A., Braun, B. R. & Geiduschek, E. P. EMBO J. 9, 2197–2205 (1990).
Jacob, F., Brenner, S. & Cuzin, F. Cold Spring Harbor Symp. quant. Biol. 28, 329–348 (1963).
Holmes, S. G. & Smith, M. M. Molec. cell. Biol. 9, 5464–5472 (1989).
Fuller, R. S., Funnell, B. E. & Kornberg, A. Cell 38, 889–900 (1984).
Dodson, M., Roberts, J., McMacken, R. & Echols, H. Proc. natn. Acad. Sci. U.S.A. 82, 4678–4682 (1985).
Funnell, B. E., Baker, T. A. & Kornberg, A. J. biol. Chem. 262, 10327–10334 (1987).
Weiss, D. S., Batut, J., Klose, K. E., Keener, J. & Kustu, S. Cell 67, 155–167 (1991).
Kustu, S., Santera, E., Keener, J., Popham, J. & Weiss, D. Microbiol. Rev. 53, 367–376 (1989).
Yang, L., Li, R., Mohr, I. J., Clark, R. & Botchan, M. R. Nature 353, 628–632 (1991).
Deb, S. W. & Tegtmeyer, P. J. Virol. 61, 3649–3654 (1987).
Dean, F. B., Dodson, M., Echols, H. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 84, 8981–8985 (1987).
Lorimer, H. E., Wang, E. H. & Prives, C. J. Virol. 65, 687–699 (1991).
Bramhill, D. & Kornberg, A. Cell 52, 743–755 (1988).
Seikimizu, K., Bramhill, D. & Kornberg, A. J. biol. Chem. 263, 7124–7130 (1988).
Seikimizu, K., Yung, B. Y.-M. & Kornberg, A. J. biol. Chem. 263, 7136–7140 (1988).
Linskens, M. H. K. & Huberman, J. A. Cell 62, 845–847 (1990).
Lue, N. F. & Kornberg, R. D. Proc. natn. Acad. Sci. U.S.A. 84, 8839–8843 (1987).
Kadonaga, J. T. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 83, 5889–5893 (1986).
Galas, A. & Schmitz, D. J. Nucleic Acids Res. 5, 3157–3170 (1978).
Diffley, J. F. X. & Cocker, J. H. Nature 357, 169–172 (1992).
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Bell, S., Stillman, B. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357, 128–134 (1992). https://doi.org/10.1038/357128a0
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DOI: https://doi.org/10.1038/357128a0
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