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New mammalian chloride channel identified by expression cloning

Nature volume 356, pages 238–241 (1992)Cite this article

Abstract

ION channels selectively permeable to chloride ions regulate cell functions as diverse as excitability and control of cell volume1–5. Using expression cloning techniques, a complementary DNA from an epithelial cell line has been isolated, sequenced and its putative structure examined by site-directed mutagenesis. This cDNA, encoding a 235-amino-acid protein, gave rise to a chloride-selective outward current when expressed in Xenopus oocytes. The expressed, outwardly rectifying chloride current was calcium-insensitive and was blocked by nucleotides applied to the cell surface. Mutation of a putative nucleotide-binding site resulted in loss of nucleotide block but incurred dependence on extracellular calcium concentration. The unusual sequence of this putative channel protein suggests a new class of ion channels not related to other previously cloned chloride channels6–11.

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Author notes

  1. Markus Paulmichl

    Present address: Department of Physiology, University of Innsbruck, Fritz-Pregistrasse 3, Innsbruck, A-6010, Austria

  2. Yi Li and Ernest Peralta: Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02115, USA

Affiliations

  1. Department of Pharmacology, Mayo Foundation, Rochester, Minnesota, 55905, USA

    Markus Paulmichl, Yi Li, Kevin Wickman, Michael Ackerman, Ernest Peralta & David Clapham

Authors
  1. Markus Paulmichl
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  6. David Clapham
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Paulmichl, M., Li, Y., Wickman, K. et al. New mammalian chloride channel identified by expression cloning. Nature 356, 238–241 (1992). https://doi.org/10.1038/356238a0

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