Abstract
To produce an immune reaction against a foreign protein usually requires purification of that protein, which is then injected into an animal. The isolation of enough pure protein is time-consuming and sometimes difficult. Here we report that such a response can also be elicited by introducing the gene encoding a protein directly into the skin of mice. This is achieved using a hand-held form of the biolistic system1–4 which can propel DNA-coated gold micro-projectiles directly into cells in the living animal3,5,6. Genetic immunization may be time- and labour-saving in producing antibodies and may offer a unique method for vaccination.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Optimized polyepitope neoantigen DNA vaccines elicit neoantigen-specific immune responses in preclinical models and in clinical translation
Genome Medicine Open Access 21 April 2021
-
Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
Cell Death & Disease Open Access 20 January 2021
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Sanford, J. C., Klein, T. M., Wolf, E. D. & Allen, N. Particulate Sci. Technol. 5, 27–37 (1987).
Armaleo, D. et al. Curr. Genet. 17, 97–103 (1990).
Sanford, J. C. et al. Technique 3, 3–16 (1991).
Sanford, J. C., Smith, R. D. & Russell, J. A. Meth. Enzym. (in the press).
Williams, R. S. et al. Proc. natn. Acad. Sci. U.S.A. 88, 2726–2730 (1991).
Johnston, S. A. et al. In Vitro cell. dev. biol. 27, 11–14 (1991).
Leavitt, J. et al. Molec. cell. Biol. 4, 1961–1969 (1984).
Boshart, M. et al. Cell 41, 521–530 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tang, Dc., DeVit, M. & Johnston, S. Genetic immunization is a simple method for eliciting an immune response. Nature 356, 152–154 (1992). https://doi.org/10.1038/356152a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/356152a0
This article is cited by
-
Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
Cell Death & Disease (2021)
-
Optimized polyepitope neoantigen DNA vaccines elicit neoantigen-specific immune responses in preclinical models and in clinical translation
Genome Medicine (2021)
-
Design and application of a newly generated bio/synthetic cryogel column for DNA capturing
Polymer Bulletin (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
