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Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures


WE have isolated a set of ligand-binding DNA sequences from a large pool of random sequence DNAs by selection and amplification in vitro, using similar methods to those described for the isolation of ligand-binding RNAs1. The ligand–DNA inter-actions are both sequence- and ligand-specific, and are dependent on proper folding of the single-stranded DNA. Some ligands led to the isolation of more DNA sequences than RNA sequences, and vice versa. Analysis of individual sequences reveals that ligand binding is DNA-specific; RNAs of identical sequence could not interact with the same ligands. Ligand-binding DNAs might be more suitable than RNAs as potential pharmacological reagents2–4 because of the greater stability of DNA. The apparent primacy of RNA in the early evolution of life5–7 may have been due to its availability rather than to its functional superiority.

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  1. Ellington, A. D. & Szostak, J. W. Nature 346, 818–822 (1990).

    Article  ADS  CAS  Google Scholar 

  2. Tuerk, C. & Gold, L., Science 249, 505–510 (1990).

    Article  ADS  CAS  Google Scholar 

  3. Abelson, J. Science 249, 488–489 (1990).

    Article  ADS  CAS  Google Scholar 

  4. Riordan, M. L. & Martin, J. C. Nature 350, 442–443 (1991).

    Article  ADS  Google Scholar 

  5. Gilbert, W. Nature 319, 618 (1986).

    Article  ADS  Google Scholar 

  6. Visser, C. M. & Kellog, R. M. J. moiec. Evol, 11, 171–187 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Orgel, L. E. & Sulston, J. E., in Prebiotic and Biochemical Evolution (eds Kimball, A. P. & Oro, J), 89–94 (North-Holland, Amsterdam, 1971).

    Google Scholar 

  8. Gyllenstein, U. B. & Erlich, H. A. Proc. natn. Acad. Sci. U.S.A. 85, 7652–7656 (1988).

    Article  ADS  Google Scholar 

  9. Arnold, F. H. Schofield, S. A. & Blanch, H. W. J. Chromatogr, 355, 1–12 (1986).

    Article  CAS  Google Scholar 

  10. Schultz, P. G., Lerner, R. A. & Benkovic, S. J. J. chem. Engng News 68, 26–40 (1990).

    Article  CAS  Google Scholar 

  11. Ellington, A. D. in Current Protocols in Molecular Biology (eds Ausubel, F. M. et al.) 2.12.1–2.12.5. (Wiley-Interscience, New York, 1987).

    Google Scholar 

  12. Green, R., Ellington, A. D., Bartel, D. P. & Szostak, J. W. Methods 2, 75–86 (1991).

    Article  CAS  Google Scholar 

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Ellington, A., Szostak, J. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature 355, 850–852 (1992).

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