Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Cloning and domain structure of the mammalian splicing factor U2AF

Nature volume 355, pages 609–614 (1992)Cite this article

Abstract

A complementary DNA clone encoding the large subunit of the essential mammalian premessenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleo protein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Green, M. R. A. Rev Cell Biol. 7, 559–599 (1991).

    Article  CAS  Google Scholar 

  2. Maniatis, T. & Reed, R. Nature 325, 673–678 (1987).

    Article  CAS  ADS  Google Scholar 

  3. Sharp, P. A. Science 235, 766–771 (1987).

    Article  CAS  ADS  Google Scholar 

  4. Guthrie, C. Science 253, 157–163 (1991).

    Article  CAS  ADS  Google Scholar 

  5. Barabino, S. M. L., Blencowe, B. J., Ryder, U., Sproat, B. S. & Lamond, A. I. Cell 63, 293–302 (1990).

    Article  CAS  Google Scholar 

  6. Kramer, A. & Utans, U. EMBO J. 10, 1503–1510 (1991).

    Article  CAS  Google Scholar 

  7. Ruskin, B., Zamore, P. D. & Green, M. R. Cell 52, 207–219 (1988).

    Article  CAS  Google Scholar 

  8. Zamore, P. D. & Green, M. R. Proc. natn Acad. Sci. U.S.A. 86, 9243–9247 (1989).

    Article  CAS  ADS  Google Scholar 

  9. Zamore, P. D. & Green, M. R. EMBO J. 10, 207–214 (1991).

    Article  CAS  Google Scholar 

  10. Gardiner-Garden, M. & Frommer, M. J. molec. Biol. 196, 261–282 (1987).

    Article  CAS  Google Scholar 

  11. Theissen, H. et al. EMBO J. 5, 3209–3217 (1986).

    Article  CAS  Google Scholar 

  12. Query, C. C. & Keene, J. D. Cell 51, 211–220 (1987).

    Article  CAS  Google Scholar 

  13. Etzerodt, M. et al. EMBO J. 7, 4311–4321 (1988).

    Article  CAS  Google Scholar 

  14. Mancebo, R., Lo, P. C. H. & Mount, S. M. Molec. cell. Biol. 10, 2492–2502 (1990).

    Article  CAS  Google Scholar 

  15. Ge, H., Zuo, P. & Manley, J. L. Cell 66, 373–382 (1991).

    Article  CAS  Google Scholar 

  16. Krainer, A. R., Mayeda, A., Kozak, D. & Binns, G. Cell 66, 383–394 (1991).

    Article  CAS  Google Scholar 

  17. Boggs, R. T., Gregor, P., Idriss, S., Belote, J. M. & McKeown, M. Cell 50, 739–747 (1987).

    Article  CAS  Google Scholar 

  18. Amrein, K. Gorman, M. & Nöthiger, R. Cell 55, 1025–1035 (1988).

    Article  CAS  Google Scholar 

  19. Goralski, T. J., Edström, J-E. & Baker, B. S. Cell 56, 1011–1018 (1989).

    Article  CAS  Google Scholar 

  20. Voelker, R. A., Gibson, W., Graves, J. P., Sterling, J. F. & Eisenberg, M. T. Molec. cell. Biol. 11, 894–905 (1991).

    Article  CAS  Google Scholar 

  21. Chou, T-B., Zachar, Z. & Bingham, P. M. EMBO J. 6, 4095–4104.

  22. Dreyfus, G., Swanson, M. S. & Pin̄ol-Roma, S. Trends biochem. Sci. 13, 86–91 (1988).

    Article  Google Scholar 

  23. Kenan, D. J., Query, C. C. & Keene, J. D. Trends biochem Sci. 16, 214–220 (1991).

    Article  CAS  Google Scholar 

  24. Garcia-Blanco, M. A., Jamison, S. & Sharp, P. A. Genes Dev. 3, 1874–1886 (1989).

    Article  CAS  Google Scholar 

  25. Zamore, P. D., Zapp, M. L. & Green, M. R. Nature 348, 485–486 (1991).

    Article  ADS  Google Scholar 

  26. Scherly, D., Dathan, N. A., Boelens, W., van Venrooij, W. J. & Mattaj, I. W. EMBO J. 9, 3675–3681.

  27. Reed, R. Genes Dev. 3, 2113–2123 (1989).

    Article  CAS  Google Scholar 

  28. Mullen, M. P., Smith, C. W. J., Patton, J. G. & Nadal-Ginard, B. Genes Dev. 5, 642–655 (1991).

    Article  CAS  Google Scholar 

  29. Smith, C. W. J., Porro, E., Patton, J. G. & Nadal-Ginard, B. Nature 342, 243–247 (1989).

    Article  CAS  ADS  Google Scholar 

  30. Zillmann, M., Zapp, M. L. & Berget, S. M. Molec. cell. Biol. 8, 814–821 (1988).

    Article  CAS  Google Scholar 

  31. Bindereif, A. & Green, M. R. EMBO J. 6, 2415–2424 (1987).

    Article  CAS  Google Scholar 

  32. Li, H. & Bingham, P. M. Cell, 67, 335–342 (1991).

    Article  CAS  Google Scholar 

  33. Carmo-Fonseca M. et al. EMBO J. 10, 195–207 (1990).

    Article  Google Scholar 

  34. Smith, C. W. J., Patton, J. G. & Nadal-Ginard, B. A. Rev. Genet. 23, 527–577 (1989).

    Article  CAS  Google Scholar 

  35. Baker, B. S. Nature 340, 521–524 (1989).

    Article  CAS  ADS  Google Scholar 

  36. Hedley, M. L. & Maniatis T. Cell 65, 579–586 (1991).

    Article  CAS  Google Scholar 

  37. Aebersold, R. H., Leavitt, J., Saavedra, R. A., Hood, L. E. & Kent, S. B. H. Proc. natn. Acad. Sci. U.S.A. 84, 6970–6974 (1987).

    Article  CAS  ADS  Google Scholar 

  38. Smith, D. B. & Johnson, K. S. Gene 67, 31–40 (1988).

    Article  CAS  Google Scholar 

  39. Lin, Y. S. & Green, M. R. Cell 64, 971–981 (1991).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts, 02138, USA

    Phillip D. Zamore

  2. Laboratory of Molecular and Cellular Physiology, Harvard Medical School, Boston, Massachusetts, 02115, USA

    James G. Patton

  3. Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts, 01605, USA

    Michael R. Green

Authors
  1. Phillip D. Zamore
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James G. Patton
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael R. Green
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zamore, P., Patton, J. & Green, M. Cloning and domain structure of the mammalian splicing factor U2AF. Nature 355, 609–614 (1992). https://doi.org/10.1038/355609a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/355609a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing