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A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II

Nature volume 355, pages 464–467 (1992)Cite this article

Abstract

RAP30/74 (also known as TFIIF (refs 1, 2), βΓ (ref. 3) and FC (ref. 4)) is one of several general factors required for initiation by RNA polymerase II (reviewed in refs 5–7). The small RAP30 subunit of RAP30/74 binds directly to polymerase and appears structurally and functionally homologous to bacterial sigma factors in their RNA polymerase-binding region8–10. RAP30/74 or recombinant RAP30 suppresses nonspecific binding of RNA polymerase II to DNA (refs 10,11) and is required for RNA polymerase II to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID, TFIIA and TFIIB (refs 2,12, 13); both RAP30 and RAP74 are physical components of the preinitiation complex2. A complementary DNA encoding human RAP30 has been isolated8, and here we report the isolation of a cDNA encoding human RAP74. RAP30 and RAP74 produced in Escherichia coli can be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II In vitro.

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References

  1. Flores, O., Maldonado, E., Burton, Z. F., Greenblatt, J. & Reinberg, D. J. biol. Chem. 263, 10812–10816 (1988).

    CAS  PubMed  Google Scholar 

  2. Flores, O. et al. Proc. natn. Acad. Sci. U.S.A. 88, 9999–10003 (1991).

    Article  ADS  CAS  Google Scholar 

  3. Conaway, J. W. & Conaway, R. C. J. biol. Chem 264, 2357–2362 (1989).

    CAS  PubMed  Google Scholar 

  4. Katajima, S. et al. Nucleic Acids Res. 18, 4843–4849.

  5. Saltzman, A. R. & Weinmann, R. FASEB J. 3, 1723–1733 (1989).

    Article  CAS  Google Scholar 

  6. Mermelstein, F. H., Flores, O. & Reinberg, D. Biochim. biophys. Acta 1009, 1–10 (1989).

    Article  CAS  Google Scholar 

  7. Sawadogo, M. & Sentenac, A. A. Rev. Biochem. 59, 711–754 (1990).

    Article  CAS  Google Scholar 

  8. Sopta, M., Burton, Z. F. & Greenblatt, J. Nature 341, 410–414 (1989).

    Article  ADS  CAS  Google Scholar 

  9. McCracken, S. & Greenblatt, J. Science 253, 900–902 (1991).

    Article  ADS  CAS  Google Scholar 

  10. Killeen, M. T. & Greenblatt, J. Molec. cell. Biol. (in the press).

  11. Conaway, J. W. & Conaway, R. C. Science 248, 1550–1553 (1990).

    Article  ADS  CAS  Google Scholar 

  12. Conaway, R. C. Garrett, K. P., Hanley, J. P. & Conaway, J. W. Proc. natn. Acad. Sci. U.S.A. 88, 6205–6209 (1991).

    Article  ADS  CAS  Google Scholar 

  13. Buratowski, S., Sopta, M., Greenblatt, J. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 88, 7509–7513 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Sopta, M., Carthew, R. W. & Greenblatt, J. J. Biol. Chem. 260, 10353–10361 (1985).

    CAS  PubMed  Google Scholar 

  15. Burton, Z. F., Ortolan, L. G. & Greenblatt, J. EMBO J. 5, 2923–2930 (1986).

    Article  CAS  Google Scholar 

  16. Burton, Z. F., Killeen, M., Sopta, M., Ortolan, L. G. & Greenblatt, J. Molec. cell. Biol. 8, 1602–1613 (1988).

    Article  CAS  Google Scholar 

  17. Bunick, D., Zandomeni, R., Ackerman, S. & Weinmann, R. Cell 29, 877–886 (1982).

    Article  CAS  Google Scholar 

  18. Conaway, R. C. & Conaway, J. W. J. biol. Chem. 263, 2962–2968 (1988).

    CAS  PubMed  Google Scholar 

  19. Saraste, M., Sibbald, P. R. & Wittnghofer, A. Trends Biochem. Sci. 15, 430–434 (1990).

    Article  Google Scholar 

  20. Flores, O., Ha, I. & Reinberg, D. J. biol. Chem. 265, 5629–5634 (1990).

    CAS  Google Scholar 

  21. Kao, C. C. et al. Science 248, 1646–1649 (1990).

    Article  ADS  CAS  Google Scholar 

  22. Peterson, M. G., Tanese, N., Pugh, B. F. & Tjian, R. Science 248, 1625–1630 (1990).

    Article  ADS  CAS  Google Scholar 

  23. Hoffmann, A. et al. Nature 346, 387–389 (1990).

    Article  ADS  CAS  Google Scholar 

  24. Ha, I., Lane, W. S. & Reinberg, D. Nature 352, 689–695 (1991).

    Article  ADS  CAS  Google Scholar 

  25. Ohkuma, Y., Sumimoto, H., Hoffmann, A., Shimasaki, S., Honkoshi, M. & Roeder, R. G. Nature 354, 398–401 (1991).

    Article  ADS  CAS  Google Scholar 

  26. Sumimoto, H., Ohkuma, Y., Sinn, E., Shimasaki, S., Kato, H., Horikoshi, M. & Roeder, R. G. Nature 354, 401–404 (1991).

    Article  ADS  CAS  Google Scholar 

  27. Peterson, M. G., Inostroza, J., Maxon, M. E., Flores, O., Admon, A., Reinberg, D. & Tjian, R. Nature 354, 369–373 (1991).

    Article  ADS  CAS  Google Scholar 

  28. Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12, 387–395 (1984).

    Article  CAS  Google Scholar 

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Author notes

  1. Joyce Li and Jack Greenblatt: Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G1L6, Canada

  2. Zachary F. Burton: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Biochemistry and Agricultural Experiment Station, Michigan State University, E. Lansing, Michigan, 48824, USA

    Ann Finkelstein, Corwin F. Kostrub, Joyce Li, David P. Chavez, Bo Qing Wang, Shi Min Fang, Jack Greenblatt & Zachary F. Burton

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  1. Ann Finkelstein
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  2. Corwin F. Kostrub
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  3. Joyce Li
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  6. Shi Min Fang
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  7. Jack Greenblatt
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Finkelstein, A., Kostrub, C., Li, J. et al. A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II. Nature 355, 464–467 (1992). https://doi.org/10.1038/355464a0

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