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Mapping T-cell receptor–peptide contacts by variant peptide immunization of single-chain transgenics

Naturevolume 355pages224230 (1992) | Download Citation

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Abstract

To test models of T-cell recognition, mice transgenic for T-cell receptor α or β chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor Vαor Vβ chains, indicating direct T-cell receptor–peptide contact, and allowing derivation of a topology for the T-cell receptor–MHC interaction. At one position on the peptide, variants transformed a homogeneous Vβ response into a very heterogeneous one.

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Author notes

    • Barbara Fazekas de St. Groth

    Present address: Clinical Immunology Center, Sydney, NSW, 2006, Australia

Affiliations

  1. Department of Microbiology and Immunology, Stanford University, Stanford, California, 94305, USA

    • Jeffrey L. Jorgensen
    • , Ursula Esser
    • , Barbara Fazekas de St. Groth
    • , Philip A. Reay
    •  & Mark M. Davis
  2. The Howard Hughes Medical Institute, Stanford University, Stanford, California, 94305, USA

    • Barbara Fazekas de St. Groth
    •  & Mark M. Davis

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https://doi.org/10.1038/355224a0

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