Abstract
To test models of T-cell recognition, mice transgenic for T-cell receptor α or β chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor Vαor Vβ chains, indicating direct T-cell receptor–peptide contact, and allowing derivation of a topology for the T-cell receptor–MHC interaction. At one position on the peptide, variants transformed a homogeneous Vβ response into a very heterogeneous one.
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Jorgensen, J., Esser, U., Fazekas de St. Groth, B. et al. Mapping T-cell receptor–peptide contacts by variant peptide immunization of single-chain transgenics. Nature 355, 224–230 (1992). https://doi.org/10.1038/355224a0
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DOI: https://doi.org/10.1038/355224a0
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