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New yeast actin-like gene required late in the cell cycle

Nature volume 355, pages 179–182 (1992)Cite this article

Abstract

ACTIN, a major cytoskeletal component of all eukaryotic cells, is one of the most highly conserved proteins. It is involved in various cellular processes such as motility, cytoplasmic streaming, chromosome segregation and cytokinesis1,2. The actin from the yeast Saccharomyces cerevisiae, encoded by the essential ACT1 gene3–5, is 89% identical to mouse cytoplasmic actin and is involved in the organization and polarized growth of the cell surface6–9. We report here the characterization of ACT29 a previously undescribed yeast split gene encoding a putative protein (391 amino acids, relative molecular mass (Mr) 44,073) that is 47% identical to yeast actin. The requirement of the ACT2 gene for vegetative growth of yeast cells and the existence of related genes in other eukaryotes indicate an important and conserved role for these actin-like proteins. Superimposition of the Act2 polypeptide onto the three-dimensional structure9,10 of known actins reveals that most of the divergence occurred in loops involved in actin polymerization, DNase I and myosin binding, leaving the core domain mainly unaffected. To our knowledge, the Act2 protein from 5. cerevisiae is the first highly divergent actin molecule described. Structural and physiological data suggest that the Act2 protein might have an important role in cytoskeletal reorganization during the cell cycle.

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References

  1. Pollard, T. D. & Cooper, J. A. A. Rev. Biochem. 555, 987–1035 (1986).

    Article  Google Scholar 

  2. Drubin, D. G. Cell Motil. Cytoskel 15, 7–11 (1990).

    CAS  Article  Google Scholar 

  3. Gallwitz, D. & Sures, I. Proc. natn. Acad. Sci. U.S.A. 77, 2546–2550 (1980).

    ADS  CAS  Article  Google Scholar 

  4. Ng, R. & Abelson, J. Proc. natn. Acad. Sci. U.S.A. 77, 3912–3916 (1980).

    ADS  CAS  Article  Google Scholar 

  5. Shortle, D., Haber, J. E. & Botstein, D. Science 217, 371–373 (1982).

    ADS  CAS  Article  Google Scholar 

  6. Kilmartin, J. V. & Adams, A. E. M. J. Cell Biol. 98, 922–933 (1984).

    CAS  Article  Google Scholar 

  7. Adams, A. E. M. & Pringle, J. R. J. Cell Biol. 98, 934–945 (1984).

    CAS  Article  Google Scholar 

  8. Novick, P. & Botstein, D. Cell 40, 405–416 (1985).

    CAS  Article  Google Scholar 

  9. Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F. & Holmes, K. C. Nature 347, 37–44 (1990).

    ADS  CAS  Article  Google Scholar 

  10. Holmes, K. C., Popp, D., Gebhard, W. & Kabsch, W. Nature 347, 44–48 (1990).

    ADS  CAS  Article  Google Scholar 

  11. Snyder, M., Elledge, S., Sweetser, D., Young, R. A. & Davis, R. W. Meth. Enzym. 159, 107–128 (1987).

    Article  Google Scholar 

  12. Legrain, P. et al. Molec. Gen. Genet. 225, 199–202 (1991).

    CAS  Article  Google Scholar 

  13. Mortimer, R. K. & Schild, D. Microbiol. Rev. 49, 181–212 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Greer, C. & Schekman, R. Molec. cell. Biol. 2, 1270–1278 (1982).

    CAS  Article  Google Scholar 

  15. Huffaker, T. C., Hoyt, M. A. & Botstein, D. A Rev. Genet. 21, 259–284 (1987).

    CAS  Article  Google Scholar 

  16. Adams, M. D. et al. Science 252, 1651–1656 (1991).

    ADS  CAS  Article  Google Scholar 

  17. Elzinga, M., Collins, J. H., Kuehl, W. M. & Adelstein, R. S. Proc. natn. Acad. Sci. USA. 70, 2687–2691 (1973).

    ADS  CAS  Article  Google Scholar 

  18. Haarer, B. K. & Pringle, J. R. Molec. cell. Biol. 7, 3678–3687 (1987).

    CAS  Article  Google Scholar 

  19. Solomon, L. R. & Rubenstein, P. A. J. biol. Chem. 262, 11382–11388 (1988).

    Google Scholar 

  20. Aktories, K. & Wegner, A. J. Cell Biol. 109, 1385–1387 (1989).

    CAS  Article  Google Scholar 

  21. Vandekerckhove, J., Deboben, A., Nassal, M. & Wieland, T. EMBO J. 4, 2815–2818 (1985).

    CAS  Article  Google Scholar 

  22. Moreno, S. & Nurse, P. Cell 61, 549–551 (1990).

    CAS  Article  Google Scholar 

  23. Surana, U. et al. Cell 65, 145–161 (1991).

    CAS  Article  Google Scholar 

  24. Ghiara, J. B. et al. Cell 65, 163–174 (1991).

    CAS  Article  Google Scholar 

  25. Langford, C. J. & Gallwitz, D. Cell 33, 519–527 (1983).

    CAS  Article  Google Scholar 

  26. Chasman, D. I. et al. Genes Dev. 4, 503–514 (1990).

    CAS  Article  Google Scholar 

  27. Dale, R. M. K., McClure, B. A. & Houchins, J. P. Plasmid 13, 31–40 (1985).

    CAS  Article  Google Scholar 

  28. Devereux, J., Haeberli, P. & Smithies, P. Nucleic Acids Res. 12, 387–395 (1984).

    CAS  Article  Google Scholar 

  29. Rothstein, R. J. Meth. Enzym. 101, 202–211 (1983).

    CAS  Article  Google Scholar 

  30. Sherman, F., Fink, G. R. & Hicks, J. B. Methods in Yeast Genetics (Cold Spring Harbor Laboratory, New York, 1986).

    Google Scholar 

  31. McKnight, S. L. & Kingsbury, R. Science 217, 316–324 (1982).

    ADS  CAS  Article  Google Scholar 

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Author notes

  1. Etienne Schwob

    Present address: Research Institute of Molecular Pathology, A-1030, Vienna, Austria

Affiliations

  1. Laboratoire de Biochimie, Institut de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique and Universite Louis Pasteur, 15 rue Rene Descartes, 67084, Strasbourg, France

    Etienne Schwob & Robert P. Martin

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  1. Etienne Schwob
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  2. Robert P. Martin
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Schwob, E., Martin, R. New yeast actin-like gene required late in the cell cycle. Nature 355, 179–182 (1992). https://doi.org/10.1038/355179a0

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