Atomic structure of single-stranded DNA bacteriophage ΦX174 and its functional implications

Nature volume 355, pages137–143(1992)Cite this article

Abstract

The mechanism of DNA ejection, viral assembly and evolution are related to the structure of bacteriophage ΦX174. The F protein forms a T = 1 capsid whose major folding motif is the eight–stranded antiparallelβ barrel found in many other icosahedral viruses. Groups of 5 G proteins form 12 dominating spikes that enclose a hydrophilic channel containing some diffuse electron density. Each G protein is a tight β barrel with its strands running radially outwards and with a topology similar to that of the F protein. The 12 'pilot' H proteins per virion may be partially located in the putative ion channel. The small, basic J protein is associated with the DNA and is situated in an interior cleft of the F protein. Tentatively, there are three regions of partially ordered DNA structure, accounting for about 12% of the total genome.

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Author notes

  1. Peter Willingmann

    Present address: Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

  2. S. Krishnaswamy

    Present address: Bioinformatics Center, Department of Biotechnology, Madurai Kamarai University, Madurai, 625 021, India

  3. Michael G. Rossmann: To whom correspondence should be addressed.

Affiliations

  1. Department of Biological Sciences, Purdue University, West Lafayette, Indiana, 47907, USA

    Robert McKenna, Di Xia, Peter Willingmann, Leodevico L. IIag, S. Krishnaswamy, Michael G. Rossmann, Norman H. Olson & Timothy S. Baker

  2. Department of Microbiology and Immunology, Center for the Health Sciences, University of Tennessee, 858 Madison Avenue, Memphis, Tennessee, 38163, USA

    Leodevico L. IIag & Nino L. Incardona

  3. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    Di Xia

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McKenna, R., Xia, D., Willingmann, P. et al. Atomic structure of single-stranded DNA bacteriophage ΦX174 and its functional implications. Nature 355, 137–143 (1992). https://doi.org/10.1038/355137a0

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