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A molecular chaperone from a thermophilic archaebacterium is related to the eukaryotic protein t-complex polypeptide-1

Nature volume 354, pages 490–493 (1991)Cite this article

Abstract

THERE is evidence to suggest that components of archaebacteria are evolutionarily related to cognates in the eukaryotic cytosol–7. We postulated that the major heat-shock protein of the thermophilic archaebacterium, Sulfolobus shibatae, is a molecular chaperone and that it is related to an as-yet unidentified chaperone component in the eukaryotic cytosol. Acquired thermotolerance in S. shibatae correlates with the predominant synthesis of this already abundant protein, referred to as thermophilic factor 55 (TF55; ref. 8). TF55 is a homo-oligomeric complex of two stacked 9-membered rings, closely resembling the 7-membered-ring complexes of the chaperonins, groEL, hsp60 and Rubisco-binding protein9–15. The TF55 complex binds unfolded polypeptides in vitro and has ATPase activity—features consistent with its being a molecular chaperone16,17. The primary structure of TF55, however, is not significantly related to the chaperonins. On the other hand, it is highly homologous (36–40% identity) to a ubiquitous eukaryotic protein, t-complex polypeptide-1 (TCP1; refs 18–20). In Saccharomyces cerevisiae, TCP1 is an essential protein that may play a part in mitotic spindle formation21. We suggest that TF55 in archaebacteria and TCP1 in the eukaryotic cytosol are members of a new class of molecular chaperones.

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Authors and Affiliations

  1. Howard Hughes Medical Institute and Department of Genetics, Yale School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510, USA

    Jonathan D. Trent & Arthur L. Horwich

  2. Program in Cellular Biochemistry and Biophysics, Rockefeller Research Laboratory, Sloan–Kettering Institute, 1275 York Avenue, New York, New York, 10021, USA

    Elmar Nimmesgern & F.-Ulrich Hartl

  3. Brookhaven National Laboratory, Biology Department, Upton, New York, 11973, USA

    Joseph S. Wall

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Trent, J., Nimmesgern, E., Wall, J. et al. A molecular chaperone from a thermophilic archaebacterium is related to the eukaryotic protein t-complex polypeptide-1. Nature 354, 490–493 (1991). https://doi.org/10.1038/354490a0

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