Letter | Published:

Cloning of cDNA for the glutamate-binding subunit of an NMDA receptor complex

Nature volume 354, pages 7073 (07 November 1991) | Download Citation

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Abstract

THE amino acids L-glutamic and L-aspartic acids form the most widespread excitatory transmitter network in mammalian brain1,2. The excitation produced by L-glutamic acid is important in the early development of the nervous system3,4, synaptic plasticity and memory formation5,6, seizures7,8 and neuronal degeneration9,10. The receptors activated by L-glutamic acid are a target for therapeutic intervention in neurodegenerative diseases, brain ischaemia and epilepsy. There are two types of receptors for the excitatory amino acids, those that lead to the opening of cation-selective channels and those that activate phospholipase C (ref. 11). The receptors activating ion channels are NMDA (N-methyl-D-aspartate) and kainate/AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive receptors. The complementary DNAs for the kainate/AMPA receptor12,13 and for the meta-botropic receptor14 have been cloned. We report here on the isolation and characterization of a protein complex of four major proteins that represents an intact complex of the NMDA receptor ion channel and on the cloning of the cDNA for one of the subunits of this receptor complex, the glutamate-binding protein.

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Author information

Affiliations

  1. Department of Pharmacology and Toxicology, 2099 Constant Avenue, West Campus, University of Kansas, Lawrence, Kansas 66047, USA

    • Keshava N. Kumar
    • , Nanda Tilakaratne
    • , Peter S. Johnson
    • , Annette E. Alien
    •  & Elias K. Michaelis
  2. Center for Biomedical Research, 2099 Constant Avenue, West Campus, University of Kansas, Lawrence, Kansas 66047, USA

    • Keshava N. Kumar
    •  & Elias K. Michaelis
  3. Present address: Department of Psychiatry, Medical College of Pennsylvania, EPPI, 3200 Henry Avenue, Philadelphia, Pennsylvania 19129, USA.

    • Nanda Tilakaratne
  4. To whom correspondence should be addressed.

    • Elias K. Michaelis

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https://doi.org/10.1038/354070a0

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