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Conservation of polymorphic simple sequence loci in cetacean species

Nature volume 354, pages 6365 (07 November 1991) | Download Citation

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Abstract

LENGTH polymorphisms within simple-sequence loci occur ubiquitously in non-coding eukaryotic DNA and can be highly informative in the analysis of natural populations1–4. Simple-sequence length polymorphisms (SSLP) in the long-finned pilot whale Globicephala melas (Delphinidae) have provided useful information on the mating system as well as on the genetic structure of populations5. We have therefore tested whether the polymerase chain reaction primers designed for Globicephala could also be used to uncover variability in other whale species. Homologous loci could indeed be amplified from a divers range of whales, including all toothed (Odontoceti) and baleen whales (Mysticeti) tested. Cloning and sequencing these loci from 11 different species revealed an unusually high conservation of sequences flanking the simple-sequence stretches, averaging 3.2% difference over 35–40 Myr. This represents the lowest divergence rate for neutral nucleotide positions found for any species group so far and raises the possible need for a re-evaluation of the age of the modern whales. On the other hand, the high conservation of non-coding sequences in whales simplifies the application of SSLP DNA fingerprinting in cetacean species, as primers designed for one species will often uncover variability in other species.

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Author information

Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität Munchen, Maria-Wardstrasse 1a, 8000 München 19, Germany

    • Christian Schlotteröer
    • , Bill Amos
    •  & Diethard Tautz
  2. University of Cambridge, Department of Genetics, Downing Street, Cambridge CB2 3EH, UK

    • Bill Amos
  3. Present address: Zoologisches Institut der Universität München, Luisenstrasse 14, 8000 München 2, Germany.

    • Christian Schlotteröer
    •  & Diethard Tautz

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https://doi.org/10.1038/354063a0

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