Abstract
REMOVAL of introns from eukaryotic nuclear messenger RNA precursors is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of four small nuclear ribonu-cleoprotein particles (Ul, U2, U5, and U4/U6 snRNPs) and auxiliary protein factors1,2. We have begun a genetic analysis of mammalian U2 snRNA by making second-site mutations in a suppressor U2 snRNA3. Here we find that several mutations in the 5′ end of U2 (nucleotides 3–8) are deleterious and that one of these can be rescued by compensatory base changes in the 3′ end of U6 (nucleotides 92–95). The results demonstrate genetically that the base-pairing interaction between U2 (nucleotides 37–11) and U6 snRNA (nucleotides 87–95), originally proposed on the basis of psoralen photocrosslinking experiments4, can influence the efficiency of mRNA splicing in mammals. The U2/U6 interac-tion in yeast, however, is fairly tolerant to mutation5 (D. J. Field and J. D. Friesen, personal communication), emphasizing the potential for facultative RNA interactions within the spliceosome.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Steitz, J. A. et al. in Structure and Function of Major and Minor snRNPs (ed. Birnsteil, M. L.) 115–154 (Springer, Heidelberg, 1988).
Lührmann, R. in Structure and Function of Major and Minor snRNPs (ed. Birnsteil, M. L.) 71–99 (Springer, Heidelberg, 1988).
Zhuang, Y. & Weiner, A. M. Genes Dev. 3, 1545–1552 (1989).
Hausner, T.-P., Giglio, L. M. & Weiner, A. M. Genes Dev. 4, 2146–2156 (1990).
Shuster, E. O. & Guthrie, C. Nature 345, 270–273 (1990).
Wu, J. & Manley, J. L. Genes Dev. 3, 1553–1561 (1989).
Yuo, C.-Y. & Weiner, A. M. Genes Dev. 3, 697–707 (1989).
Wu, J. & Manley, J. L. Nature 352, 818–821 (1991).
Sussman, J. L., Holbrook, S. R., Warrant, R. W., Church, G. M., & Kim, S.-H. J. molec. Biol. 123, 607–630 (1978).
Black, D. L., Chabot, B. & Steitz, J. A. Cell 42, 737–750 (1985).
Krainer, A. R. & Maniatis, T. Cell 42, 725–736 (1985).
Frendewey, D., Krämer, A. & Keller, W. Cold Spring Harbor Symp. quant. Biol. 52, 287–298 (1987).
Pan, Z.-Q. & Prives, C. Genes Dev. 3, 1887–1898 (1989).
Lamond, A. I., Sproat, B., Ryder, U. & Hamm, J. Cell 58, 383–390 (1989).
Hamm, J., Dathan, N. A. & Mattaj, I. W. Cell 59, 159–169 (1989).
Blencowe, B. J., Sproat, B. S., Ryder, U., Barabino, S. & Lamond, A. I. Cell 59, 531–539 (1989).
Vankan, P., McGuigan, C. & Mattaj, I. W. EMBO J. 9, 3397–3404 (1990).
Fabrizio, P., McPheeters, D. S. & Abelson, J. Genes Dev. 3, 2137–2150 (1989).
Fabrizio, P. & Abelson, J. Science 250, 404–409 (1991).
Madhani, H. D., Bordonne, R. & Guthrie, C. Genes Dev. 4, 2264–2277 (1991).
Guthrie, C. & Patterson, B. A. Rev. Genet. 22, 387–419 (1988).
Company, M., Arenas, J. & Abelson, J. Nature 349, 487–493 (1991).
Schwer, B. & Guthrie, C. Nature 349, 494–499 (1991).
Pikielny, C. W., Rymond, B. C. & Rosbash, M. Nature 324, 341–345 (1986).
Cheng, C.-C. & Abelson, J. Genes Dev. 1, 1014–1027 (1987).
Lamond, A., Konarska, M. M., Grabowski, P. J. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 85, 411–415 (1988).
Kunkel, T. A. Proc. natn. Acad. Sci. U.S.A. 82, 488–492 (1985).
Kunkel, G. R. & Pederson, T. Genes Dev. 2, 196–204 (1988).
Santa Lucia, J., Kierzek, R. & Turner, D. H. Biochemistry (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Datta, B., Weiner, A. Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing. Nature 352, 821–824 (1991). https://doi.org/10.1038/352821a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/352821a0
This article is cited by
-
Detection technologies for RNA modifications
Experimental & Molecular Medicine (2022)
-
Crystal structures of the Lsm complex bound to the 3′ end sequence of U6 small nuclear RNA
Nature (2014)
-
Splicing double: insights from the second spliceosome
Nature Reviews Molecular Cell Biology (2003)
-
RNA:RNA interactions in the spliceosome
Molecular Biology Reports (1993)
-
Spliced leader RNAs from lower eukaryotes are trans- spliced in mammalian cells
Nature (1992)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.