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Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter

Nature volume 352, pages 729–731 (1991)Cite this article

Abstract

THE complementary DNA sequence encoding the cell-surface receptor for ecotropic host-range murine retroviruses (ecoR) shows that it contains 622 amino acids and 14 hydrophobic potentially membrane-spanning sequences1. Because this receptor occurs on many or all murine cells2 and is probably essential for viability of cultured fibroblasts3, its normal function might be to transport an essential metabolite. We expressed ecoR in Xenopus laevis oocytes by injecting RNA transcribed from the cloned cDNA. These oocytes specifically bound the gp70 envelope glycoprotein from an ecotropic murine leukaemia virus. An inward current was recorded electrophysiologically when a mixture of amino-acids was applied: this resulted from a stereoselective, saturable uptake of lysine, arginine and ornithine; it was independent of sodium and not substantially altered by gp70. Cysteine and homoserine were also taken up, but sodium was necessary for their transport. These properties of ecoR correspond to those of the y+ amino-acid transporter4–6. Our results demonstrate the subversion of a ubiquitous cell membrane protein, in this case a basic amino acid transporter, for use as a retroviral receptor.

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References

  1. Albritton, L. M., Tseng, L., Scadden, D. & Cunningham, J. M. Cell 57, 659–666 (1989).

    Article  CAS  PubMed  Google Scholar 

  2. Weiss, R. A. in RNA Tumor Viruses (eds Weiss, R., Teich, N., Varmus, H. & Coffin, J.) 209–260 (Cold Spring Harbor Laboratory, New York, 1984).

    Google Scholar 

  3. Handelin, B. & Kabat, D. Virology 140, 183–187 (1985).

    Article  CAS  PubMed  Google Scholar 

  4. Christensen, H. N. & Liang, M. J. biol. Chem. 241, 5542–5551 (1966).

    CAS  PubMed  Google Scholar 

  5. Christensen, H. N. Meth. Enzym. 173, 576–616 (1989).

    Article  CAS  PubMed  Google Scholar 

  6. White, M. F. Biochim. biophys. Acta 822, 355–374 (1985).

    Article  CAS  PubMed  Google Scholar 

  7. Christie, M. J., Adelman, J. P., Douglass, J. & North, R. A. Science 244, 221–224 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Kabat, D. Virology 171, 464–474 (1989).

    Article  Google Scholar 

  9. Christensen, H. N., Handgloten, M. E. & Thomas, E. L. Proc. natn. Acad. Sci. U.S.A. 63, 948–955 (1969).

    Article  ADS  CAS  Google Scholar 

  10. Christensen, H. N. Biochim. biophys. Acta 779, 255–269 (1984).

    Article  CAS  PubMed  Google Scholar 

  11. White, M. F., Gazzola, G. C. & Christensen, H. N. J. biol. Chem. 257, 4443–4449 (1982).

    CAS  PubMed  Google Scholar 

  12. Moore, J. P., Keating, J. A. M., Norton, W. A. & Sattentau, Q. J. J. Virol. 65, 1133–1140 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Hoffmann, W. J. biol. Chem. 260, 11831–11837 (1985).

    CAS  PubMed  Google Scholar 

  14. Nikawa, J., Hosaka, K., Tsukagoshi, Y. & Yamashita, S. J. biol. Chem. 265, 15996–16003 (1990).

    CAS  PubMed  Google Scholar 

  15. Tanaka, G. R. & Fink, G. R. Gene 38, 205–214 (1985).

    Article  CAS  PubMed  Google Scholar 

  16. Segal, S. & Thier, S. O. in The Metabolic Basis of Inherited Disease (eds Scriver, C. R., Beaudet, A. L., Sly, W. S. & Valle, D.) 2479–2496 (McGraw-Hill, New York, 1989).

    Google Scholar 

  17. Stevenson, M., Meier, C., Mann, A. M., Chapman, N. & Wasiak, A. Cell 53, 483–496 (1988).

    Article  CAS  PubMed  Google Scholar 

  18. Delwart, E. L. & Panganiban, A. T. J. Virol. 63, 273–280 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Rein, A. Virology 120, 251–257 (1982).

    Article  CAS  PubMed  Google Scholar 

  20. DeLarco, J. & Todaro, G. J. Cell 8, 365–371 (1976).

    Article  CAS  PubMed  Google Scholar 

  21. Sommerfelt, M. A. & Weiss, R. A. Virology 176, 58–69 (1990).

    Article  CAS  PubMed  Google Scholar 

  22. Kabat, D. in Current Top. Microbiol. Immun. 148, 1–42 (1989).

    Google Scholar 

  23. Ihle, J. H., Rein, A. & Mural, R. in Advances in Viral Oncology Vol. 4 (ed. Klein, G.) 95–137 (Raven, New York, 1984).

    Google Scholar 

  24. McGrath, M. S., Pillemer, E. Kodistra, D. & Weissman, I. L. Contemp. Top. Immunobiol. 11, 157–184 (1980).

    Article  CAS  PubMed  Google Scholar 

  25. Mitani et al. Proc. natn. Acad. Sci. U.S.A. 84, 237–240 (1987).

    Article  ADS  CAS  Google Scholar 

  26. Cianciolo, G. J., Copeland, T. D., Oroszlan, S. & Snyderman, R. Science 230, 453–455 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Kleinerman, E. S., Lachman, L. B., Knowles, R. D., Snyderman, R. & Gianciolo, G. J. J. Immun. 139, 2329–2337 (1987).

    CAS  PubMed  Google Scholar 

  28. Sitbon, M. et al. Cell 47, 851–859 (1986).

    Article  CAS  PubMed  Google Scholar 

  29. Szurek, P. F., Yuen, P. H., Jerzy, R. & Wong, P. K. Y. J. Virol. 62, 357–360 (1988).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. MacLeod, C. L., Finley, K., Kakuda, D., Kozak, C. A. & Wilkinson, M. F. Molec. cell. Biol. 10, 3663–3674 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. O'Hara, B. et al. Cell Growth Differ. 1, 119–127 (1990).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Oregon Health Sciences University, Portland, Oregon, 97201, USA

    Hao Wang & David Kabat

  2. Vollum Institute, Oregon Health Sciences University, Portland, Oregon, 97201, USA

    Michael P. Kavanaugh & R. Alan North

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  1. Hao Wang
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  2. Michael P. Kavanaugh
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  3. R. Alan North
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  4. David Kabat
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Wang, H., Kavanaugh, M., North, R. et al. Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature 352, 729–731 (1991). https://doi.org/10.1038/352729a0

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