Abstract
ALTHOUGH it is established that the CDS and CD4 co-receptors are involved in T-lymphocyte recognition and activation in the periphery, it is less clear whether these molecules participate in thymic selection events. Analysis of thymic selection in mice transgenic for T cell-receptor genes1–4 or for major histocompatibility complex (MHC) genes5, or mice injected with antibodies against CDS, CD4 or MHC molecules6–8, is consistent with the participation of CDS and CD4 in thymic selection. But antibody-mediated crosslinking of surface receptors in thymic organ cultures9 has indicated that CDS is not involved in thymic deletion. We show here that mice transgenic for a mutant MHC class I molecule that cannot interact with CDS do not delete CDS-dependent T cells reactive with the wild-type molecule. This finding unequivocally establishes that for negative selection in the thymus, CDS must interact with the same MHC class I molecule as the T cell receptor.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Loh, D. Y. et al. Cold Spring Harb. Symp. quant. Biol. 54, 147–151 (1989).
Berg, L. J. et al. Cell 58, 1035–1046 (1989).
Kaye, J. et al. Nature 341, 746–749 (1989).
Kisielow, P. et al. Nature 333, 742–746 (1988).
Kalinke, U., Arnold, B. & Hammerling, G. J. Nature 348, 642–644 (1990).
Zuniga-Pflucker, J. C., Jones, L. A., Longo, D. L. & Kruisbeck, A. M. Cold Spring Harb. Symp. quant. Biol. 54, 153–158 (1989).
MacDonald, H. R., Hengartner, H. & Pedrazzini, T. Nature 335, 174–176 (1988).
Fowlkes, B. J., Schwartz, R. H. & Pardoll, D. M. Nature 334, 620–623 (1988).
Yachelini, P., Falk, I. & Eichmann, K. J. Immun. 145, 1382–1389 (1990).
Swain, S. L. Immun. Rev. 74, 129–142 (1983).
McDonald, H. R. et al. Immun. Rev. 68, 89–115 (1982).
Veillette, A., Bookman, M. A., Horak, E. M. & Bolen, J. B. Cell 55, 301–308 (1988).
Barber, E. K. et al. Proc. natn. Acad. Sci. U.S.A. 86, 3277–3281 (1989).
Veillette, A. et al. Nature 338, 257–259 (1989).
Glaichenhaus, N. et al. Cell 64, 511–520 (1991).
Potter, T. A., Bluestone, J. A., & Rajan, T. V. J. exp. Med. 166, 956–966 (1987).
Connolly, J. M., Potter, T. A., Wormstall, E. M. & Hansen, T. H. J. exp. Med. 168, 325–341 (1988).
Potter, T. A., Rajan, T. V., Dick, R. F. & Bluestone, J. A. Nature 337, 73–75 (1989).
Connolly, J. M., Hansen, T. H., Ingold, A. L. & Potter, T. A. Proc. natn. Acad. Sci. U.S.A. 87, 2137–2141 (1990).
Salter, R. D. et al. Nature 345, 41–46 (1990).
Janeway, C. A. et al. Immun. Rev. 109, 77–92 (1989).
Bieberich, C., Scangos, G., Tanaka, K. & Jay, G. Molec. cell. Biol. 6, 1339–1342 (1986).
Yoshioka, T., Bierberich, C., Scangos, G. & Jay, G. J. Immun. 139, 3861–3867 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ingold, A., Landel, C., Knall, C. et al. Co-engagement of CD8 with the T cell receptor is required for negative selection. Nature 352, 721–723 (1991). https://doi.org/10.1038/352721a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/352721a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.