Letter | Published:

Characterization of the rough endoplasmic reticulum ribosome-binding activity

Naturevolume 352pages638640 (1991) | Download Citation



THE rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites1. A purification to apparent homogeneity of a negatively charged protein (ERpl80) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor2. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERpl80. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.

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  1. 1

    Borgese, N., Mok, W., Kreibich, G. & Sabatini, D. D. J. molec. Biol. 88, 559–580 (1974).

  2. 2

    Savitz, A. J. & Meyer, D. I. Nature 346, 540–544 (1990).

  3. 3

    Hortsch, M., Avossa, D. & Meyer, D. I. J. Cell Biol. 103, 241–253 (1986).

  4. 4

    Yoshida, H. et al. Biochem. J. 259, 811–819 (1987).

  5. 5

    Collins, P. G. & Gilmore, R. J. Cell Biol. (in the press).

  6. 6

    Kreibich, G., Ulrich, B. L. & Sabatini, D. D. J. Cell Biol. 77, 464–487 (1978).

  7. 7

    Tazawa, S. J. Biochem. 109, 89–98 (1991).

  8. 8

    Walter, P. & Blobel, G. Meth. Enzym. 96, 682–691 (1983).

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  1. Department of Biochemistry and Biophysics, University of California Medical School, San Francisco, California, 94143–0448, USA

    • Jodi M. Nunnari
    • , Deborah L. Zimmerman
    • , Stephen C. Ogg
    •  & Peter Walter


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