Letter | Published:

Characterization of the rough endoplasmic reticulum ribosome-binding activity

Naturevolume 352pages638640 (1991) | Download Citation

Subjects

Abstract

THE rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites1. A purification to apparent homogeneity of a negatively charged protein (ERpl80) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor2. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERpl80. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Borgese, N., Mok, W., Kreibich, G. & Sabatini, D. D. J. molec. Biol. 88, 559–580 (1974).

  2. 2

    Savitz, A. J. & Meyer, D. I. Nature 346, 540–544 (1990).

  3. 3

    Hortsch, M., Avossa, D. & Meyer, D. I. J. Cell Biol. 103, 241–253 (1986).

  4. 4

    Yoshida, H. et al. Biochem. J. 259, 811–819 (1987).

  5. 5

    Collins, P. G. & Gilmore, R. J. Cell Biol. (in the press).

  6. 6

    Kreibich, G., Ulrich, B. L. & Sabatini, D. D. J. Cell Biol. 77, 464–487 (1978).

  7. 7

    Tazawa, S. J. Biochem. 109, 89–98 (1991).

  8. 8

    Walter, P. & Blobel, G. Meth. Enzym. 96, 682–691 (1983).

Download references

Author information

Affiliations

  1. Department of Biochemistry and Biophysics, University of California Medical School, San Francisco, California, 94143–0448, USA

    • Jodi M. Nunnari
    • , Deborah L. Zimmerman
    • , Stephen C. Ogg
    •  & Peter Walter

Authors

  1. Search for Jodi M. Nunnari in:

  2. Search for Deborah L. Zimmerman in:

  3. Search for Stephen C. Ogg in:

  4. Search for Peter Walter in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/352638a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.