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Phosphorylation-regulated CI− channel in CHO cells stably expressing the cystic fibrosis gene

Nature volume 352, pages 628–631 (1991)Cite this article

Abstract

A CYCLIC AMP-stimulated choride conductance appears when the cystic fibres is gene is expressed in non-epithelial cells by infection with recombinant viruses1,2. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia2–4, but control of this channel by phosphorylation has not been directly demonstrated. Here we report the appearance of the low-conductance Cl- channel in Chinese hamster ovary cells after stable transfection with the cystic fibres is gene. The channel is regulated on-cell by membrane-permeant analogues of cAMP and off-cell by protein kinases A and C and by alkaline phosphatase. These results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.

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Authors and Affiliations

  1. Department of Physiology, McGill University, 3655 Drummond Street, Montreal, Quebec, H3G 1Y6, Canada

    Joseph A. Tabcharani & John W. Hanrahan

  2. Research Institute, Hospital for Sick Children, and University of Toronto, Toronto, Ontario, M5G 1X8, Canada

    Xiu-Bao Chang & John R. Riordan

Authors
  1. Joseph A. Tabcharani
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  2. Xiu-Bao Chang
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  3. John R. Riordan
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  4. John W. Hanrahan
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Tabcharani, J., Chang, XB., Riordan, J. et al. Phosphorylation-regulated CI− channel in CHO cells stably expressing the cystic fibrosis gene. Nature 352, 628–631 (1991). https://doi.org/10.1038/352628a0

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