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Phosphorylation-regulated CI channel in CHO cells stably expressing the cystic fibrosis gene

Naturevolume 352pages628631 (1991) | Download Citation

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Abstract

A CYCLIC AMP-stimulated choride conductance appears when the cystic fibres is gene is expressed in non-epithelial cells by infection with recombinant viruses1,2. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia2–4, but control of this channel by phosphorylation has not been directly demonstrated. Here we report the appearance of the low-conductance Cl- channel in Chinese hamster ovary cells after stable transfection with the cystic fibres is gene. The channel is regulated on-cell by membrane-permeant analogues of cAMP and off-cell by protein kinases A and C and by alkaline phosphatase. These results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.

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Affiliations

  1. Department of Physiology, McGill University, 3655 Drummond Street, Montreal, Quebec, H3G 1Y6, Canada

    • Joseph A. Tabcharani
    •  & John W. Hanrahan
  2. Research Institute, Hospital for Sick Children, and University of Toronto, Toronto, Ontario, M5G 1X8, Canada

    • Xiu-Bao Chang
    •  & John R. Riordan

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https://doi.org/10.1038/352628a0

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