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Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico

Abstract

Concerns have been raised about the potential effects of transgenic introductions on the genetic diversity of crop landraces and wild relatives in areas of crop origin and diversification, as this diversity is considered essential for global food security. Direct effects on non-target species1,2, and the possibility of unintentionally transferring traits of ecological relevance onto landraces and wild relatives have also been sources of concern3,4. The degree of genetic connectivity between industrial crops and their progenitors in landraces and wild relatives is a principal determinant of the evolutionary history of crops and agroecosystems throughout the world5,6. Recent introductions of transgenic DNA constructs into agricultural fields provide unique markers to measure such connectivity. For these reasons, the detection of transgenic DNA in crop landraces is of critical importance. Here we report the presence of introgressed transgenic DNA constructs in native maize landraces grown in remote mountains in Oaxaca, Mexico, part of the Mesoamerican centre of origin and diversification of this crop7,8,9.

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Figure 1: PCR amplification of DNA from the maize-specific alpha zein protein gene (top panel) and the CMV p-35S promoter (centre and bottom panels).
Figure 2: Inverse PCR sequences of flanking regions adjacent to the CMV p-35S in landraces (A2, A3 and B3) and in Diconsa seed (K1).

Notes

  1. 1.

    For communications arising from this paper, see Nature advance online publication, 4 April 2002; DOI 10.1038/nature738; DOI 10.1038/nature739 and; DOI 10.1038/nature740.

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Acknowledgements

We thank the Union de Comunidades Zapoteco Chinanteca (UZACHI) for access to their field laboratory, Y. Lara (Estudios Rurales y Asesoría, Oaxaca) for facilitation, A. King for Peruvian maize samples and CIMMYT maize germplasm bank for the historical control.

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Correspondence to Ignacio H. Chapela.

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Quist, D., Chapela, I. Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico. Nature 414, 541–543 (2001). https://doi.org/10.1038/35107068

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