The p53 protein has a two-pronged approach to keeping tumours at bay: by modulating the transcription of genes involved in the cell cycle or apoptosis, it can either inhibit cell growth or stimulate cell death. Several genes have been implicated in the path to apoptosis, and two papers in Molecular Cell now report the identification of a new target — the p53 upregulated modulator of apoptosis, or PUMA for short.
The two groups used different strategies to identify PUMA. Yu et al . were exploring the roles of other known apoptotic targets — Bax, Noxa and p53AIP1 — in human colorectal cancer cells. They found that expression of these three genes was neither early nor strong enough to account for the observed apoptosis, so they looked for other candidates by the serial analysis of gene expression (SAGE) technique. Nakano and Vousden, on the other hand, used microarrays to compare gene-expression patterns in the presence and absence of p53.
Although there are differences in the transcription products identified by the two groups, the reported functional and biochemical properties of PUMA are nearly identical. The PUMA promoter contains two consensus p53-binding sites, BS1 and BS2, although BS2 seems to be the main p53-responsive element. PUMA also contains a Bcl-2 homology 3 (BH3) domain, a conserved motif found in many pro-apoptotic members of the Bcl-2 family.
Such 'BH3-only' proteins are known to form heterodimers, leading to the release of cytochrome c from the mitochondria. This activates an apoptotic cascade involving caspase-9/Apaf-1 and caspase-3. So could human PUMA be involved in this pathway? Both groups found that human PUMA indeed localizes to the mitochondria, and used immunoprecipitation to show that PUMA binds to Bcl-2. Yu et al. also showed that PUMA interacts with Bcl-xL, which is ubiquitously expressed in many colon cancer cells. The PUMA BH3 domain is essential for its interactions with both Bcl-2 and Bcl-xL.
Finally, the two groups showed that expression of PUMA leads to extremely rapid cell death in vitro. Nakano and Vousden showed that this death was accompanied by the release of cytochrome c and the cleavage of procaspase-9 and procaspase-3 to their activated forms. They also used antisense oligonucleotides against PUMA to confirm its contribution to apoptosis in a p53-inducible cell line.
What next, then, for PUMA? As Yu et al. point out, we need to know PUMA's normal function and whether it is really necessary for apoptosis. Because PUMA leads to such a rapid and profound apoptosis, they also raise the tantalising possibility that it might one day be considered as a substitute for p53 in cancer gene therapy.
References
ORIGINAL RESEARCH PAPERS
Yu, J. et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 7, 673–682 (2001) [ Contents page]
Nakano, K. & Vousden, K. H. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 7, 683 –694 (2001) [ Contents page]
FURTHER READING
Vousden, K. H. p53: death star. Cell 103, 691–694 (2000)
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- JOAN S. BRUGGE
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HARVARD MEDICAL SCHOOL, BOSTON, MA, USA
- PASCALE COSSART
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INSTITUT PASTEUR, PARIS, FRANCE
- GIDEON DREYFUSS
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UNIVERSITY OF PENNSYLVANIA, PHILADELPHIA, PA, USA
- PAMELA GANNON
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CELL AND MOLECULAR BIOLOGY ONLINE
- JEAN GRUENBERG
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UNIVERSITY OF GENEVA, SWITZERLAND
- ULRICH HARTL
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MAX-PLANCK-INSTITUTE, MARTINSRIED, GERMANY
- NOBUTAKA HIROKAWA
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UNIVERSITY OF TOKYO, JAPAN
- STEPHEN P. JACKSON
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WELLCOME/CRC INSTITUTE, CAMBRIDGE, UK
- ROBERT JENSEN
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JOHNS HOPKINS UNIVERSITY, BALTIMORE, MD, USA
- VICKI LUNDBLAD
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BAYLOR COLLEGE OF MEDICINE, HOUSTON, TX, USA
- TONY PAWSON
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SAMUEL LUNENFELD RESEARCH INSTITUTE, TORONTO, CANADA
- NORBERT PERRIMON
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HARVARD MEDICAL SCHOOL, BOSTON, MA, USA
- THOMAS D. POLLARD
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THE SALK INSTITUTE, LA JOLLA, CA, USA
- JOHN C. REED
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THE BURNHAM INSTITUTE, LA JOLLA, CA, USA
- KAREN VOUSDEN
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NATIONAL CANCER INSTITUTE, FREDERICK, MD, USA
- JOHN WALKER
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MRC DUNN HUMAN NUTRITION UNIT, CAMBRIDGE, UK
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Mitchell, A. Death by PUMA. Nat Rev Mol Cell Biol 2, 319 (2001). https://doi.org/10.1038/35073021
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DOI: https://doi.org/10.1038/35073021