Abstract
Most organisms, from cyanobacteria to mammals, use circadian clocks to coordinate their activities with the natural 24-h light/dark cycle. The clock proteins of Drosophila and mammals exhibit striking homology but do not show similarity with clock proteins found so far from either cyanobacteria or Neurospora1,2. Each of these organisms uses a transcriptionally regulated negative feedback loop in which the messenger RNA levels of the clock components cycle over a 24-h period. Proteins containing PAS domains are invariably found in at least one component of the characterized eukaryotic clocks1. Here we describe ADAGIO1 (ADO1), a gene of Arabidopsis thaliana that encodes a protein containing a PAS domain. We found that a loss-of-function ado1 mutant is altered in both gene expression and cotyledon movement in circadian rhythmicity. Under constant white or blue light, the ado1 mutant exhibits a longer period than that of wild-type Arabidopsis seedlings, whereas under red light cotyledon movement and stem elongation are arrhythmic. Both yeast two-hybrid and in vitro binding studies show that there is a physical interaction between ADO1 and the photoreceptors CRY1 and phyB. We propose that ADO1 is an important component of the Arabidopsis circadian system.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Accession codes
References
Dunlap, J. C. Molecular basis for circadian clocks. Cell 96, 271–290 (1999).
Lakin-Thomas, P. L. Circadian rhythms: new functions for old clock genes? Trends Genet. 16, 135–142 (2000).
Millar, A. Biological clocks in Arabidopsis thaliana. New Phytol. 141, 175–197 (1999).
Somers, D. The physiology and molecular bases of the plant circadian clock. Plant Physiol. 121, 9–19 (1999).
Wang, Z. & Tobin, E. Constitutive expression of the circadian clock associated 1 (CCA1) gene disrupts circadian rhythms and suppresses its own expression. Cell 93, 1207–1217 (1998).
Schaffer, R. et al. The late elongated hypocotyl mutation of Arabidopsis disrupts circadian rhythms and the photoperiodic control of flowering. Cell 93, 1219–1229 (1998).
Fowler, S. et al. GIGANTEA: a circadian clock-controlled gene that regulates photoperiodic flowering in Arabidopsis and encodes a protein with several possible membrane-spanning domains. EMBO J. 18, 4679–4688 (1999).
Sugano, S., Andronis, C., Ong, M. S., Green, R. M. & Tobin, E. M. The protein kinase CK2 is involved in regulation of circadian rhythms in Arabidopsis. Proc. Natl Acad. Sci. USA 96, 12362–12366 (1999).
Park, D. H. et al. Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science 1579–1582 (1999).
Somers, D., Devlin, P. & Kay, S. Phytochromes and cryptochromes in the entrainment of the Arabidopsis circadian clock. Science 282, 488–490 (1998).
Cashmore, A. R., Jarillo, J. A., Wu, Y. J. & Liu, D. Cryptochromes: blue light receptors for plants and animals. Science 284, 760–765 (1999).
Okamura, H. et al. Photic induction of mPer1 and mPer2 in cry-deficient mice lacking a biological clock. Science 286, 2531–2534 (1999).
Griffin, E. A., Staknis, D. & Weitz, C. J. Light-independent role of CRY1 and CRY2 in the mammalian circadian clock. Science 286, 768–771 (1999).
Kume, K. et al. mCRY1 and mCRY2 are essential components of the negative limb of the circadian clock feedback loop. Cell 98, 193–205 (1999).
Somers, D. E., Schultz, T. F., Milnamow, M. & Kay, S. A. ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis. Cell 101, 319–329 (2000).
Kiyosue, T. & Wada, M. LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in Arabidopsis. Plant J. 23, 807–815 (2000).
Nelson, D. C., Lasswell, J., Rogg, L. E., Cohen, M. A. & Bartel, B. FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis. Cell 101, 331–340 (2000).
Ballario, P. et al. White collar-1, a central regulator of a blue light responses in Neurospora, is a zinc finger protein. EMBO J. 15, 1650–1657 (1996).
Crosthwaite, S. K., Dunlap, J. C. & Loros, J. J. Neurospora wc-1 and wc-2: transcription, photoresponses, and the origins of circadian rhythmicity. Science 276, 763–769 (1997).
Christie, J. M. et al. NPH1: a flavoprotein with the properties of a photoreceptor for phototropism. Science 282, 1698–1701 (1998).
Jarillo, J. A., Ahmad, M. & Cashmore, A. R. NPL1 (Accession number AF053941): a second member of the NPH serine/threonine kinase family of Arabidopsis. Plant Physiol. 117, 719 (1998).
Taylor, B. L. & Zhulin, I. B. PAS domains: internal sensors of oxygen, redox potential, and light. Microbiol. Mol. Biol. Rev. 63, 479–506 (1999).
Patton, E. E., Willems, A. R. & Tyers, M. Combinatorial control in ubiquitin-dependent proteolysis: don’t Skp the F-box hypothesis. Trends Genet. 14, 236–243 (1998).
Adams, J., Kelso, R. & Cooley, L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 10, 17–24 (2000).
Carpenter, C. D., Kreps, J. A. & Simon, A. E. Genes encoding glycine-rich Arabidopsis thaliana proteins with RNA-binding motifs are influenced by cold treatment and an endogenous circadian rhythm. Plant Physiol. 104, 1015–25 (1994).
Dowson-Day, M. & Millar, A. Circadian dysfunction causes aberrant hypocotyl elongation patterns in Arabidopsis. Plant J. 17, 63–71 (1999).
Hicks, K. A. et al. Conditional circadian dysfunction of the Arabidopsis early-flowering 3 mutant. Science 274, 790–792 (1996).
Ballario, P. & Macino, G. White collar proteins: PASsing the light signal in Neurospora crassa. Trends Microbiol. 5, 458–462 (1997).
Ruf, T. The Lomb–Scargle periodogram in biological rhythm research: analysis of incomplete and unequally spaced time-series. Biol. Rhythm Res. 30, 178–201 (1999).
Acknowledgements
We thank N. Bonini for comments on the manuscript. This work was supported by grants from the NIH and the DOE to A.R.C., from NSF to J.R.E., and a NATO fellowship awarded to J.C.
Author information
Authors and Affiliations
Corresponding author
Supplementary information
Rights and permissions
About this article
Cite this article
Jarillo, J., Capel, J., Tang, RH. et al. An Arabidopsis circadian clock component interacts with both CRY1 and phyB. Nature 410, 487–490 (2001). https://doi.org/10.1038/35068589
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/35068589
This article is cited by
-
Characterizing the circadian connectome of Ocimum tenuiflorum using an integrated network theoretic framework
Scientific Reports (2023)
-
ZEITLUPE enhances expression of PIF4 and YUC8 in the upper aerial parts of Arabidopsis seedlings to positively regulate hypocotyl elongation
Plant Cell Reports (2021)
-
Molecular basis of heading date control in rice
aBIOTECH (2020)
-
Circadian oscillator proteins across the kingdoms of life: structural aspects
BMC Biology (2019)
-
Enhancement of hypocotyl elongation by LOV KELCH PROTEIN2 production is mediated by auxin and phytochrome-interacting factors in Arabidopsis thaliana
Plant Cell Reports (2016)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
