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Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake

Nature volume 409, pages 346349 (18 January 2001) | Download Citation

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Abstract

Frequently, crop plants do not take up adequate amounts of iron from the soil, leading to chlorosis, poor yield and decreased nutritional quality. Extremely limited soil bioavailability of iron has led plants to evolve two distinct uptake strategies: chelation, which is used by the world's principal grain crops1,2; and reduction, which is used by other plant groups3,4,5. The chelation strategy involves extrusion of low-molecular-mass secondary amino acids (mugineic acids) known as ‘phytosiderophores’, which chelate sparingly soluble iron6. The Fe(iii)-phytosiderophore complex is then taken up by an unknown transporter at the root surface7,8. The maize yellow stripe1 (ys1) mutant is deficient in Fe(iii)-phytosiderophore uptake7,8,9,10, therefore YS1 has been suggested to be the Fe(iii)-phytosiderophore transporter. Here we show that ys1 is a membrane protein that mediates iron uptake. Expression of YS1 in a yeast iron uptake mutant restores growth specifically on Fe(iii)-phytosiderophore media. Under iron-deficient conditions, ys1 messenger RNA levels increase in both roots and shoots. Cloning of ys1 is an important step in understanding iron uptake in grasses, and has implications for mechanisms controlling iron homeostasis in all plants.

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Acknowledgements

We are indebted to S. Kawai for the gift of mugineic acid and to M. L. Guerinot for the gift of IRT1 cDNA. This work was supported by a USDA NRICGP Plant Responses to the Environment Award to ELW and an NIH Award to SLD.

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Author notes

    • Catherine Curie
    •  & Zivile Panaviene

    These authors contributed equally to this work

Affiliations

  1. *Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5004), Institut National de la Recherche Agronomique, Université Montpellier 2 et École Nationale Supérieure d'Agronomie, Place Viala, F-34060 Montpellier cedex 1, France

    • Catherine Curie
    • , Clarisse Loulergue
    •  & Jean-Francois Briat
  2. ‡Biology Department, University of Massachusetts, Amherst, Massachusetts 01003 , USA

    • Zivile Panaviene
    •  & Elsbeth L. Walker
  3. §Molecular, Cellular & Developmental Biology Department, Yale University, New Haven, Connecticut 06520-8104, USA

    • Stephen L. Dellaporta

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Correspondence to Elsbeth L. Walker.

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https://doi.org/10.1038/35053080

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