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Activation of the β1 isozyme of phospholipase C by α subunits of the Gq class of G proteins

Nature volume 350, pages 516–518 (1991)Cite this article

Abstract

MANY hormones, neurotransmitters and growth factors, on binding to G protein-coupled receptors or receptors possessing tyrosine kinase activity, increase intracellular levels of the second messengers inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. This is due to activation of phosphoinositide-specific phospho-lipase(s) C (PLC), the isozymes of which are classified into groups, α, β, γ and δ (refs 1, 2). The β, γ and δ groups themselves contain PLC isozymes which have both common and unique structural domains3. Only the γl isozyme has been implicated in a signal transduction mechanism4. This involves association with, and tyrosine phosporylation by, the ligand-bound epidermal growth factor and platelet-derived growth factor receptors5–7, probably by means of the PLC-γl-specific src homology (SH2) domain8. Because EGF receptor-mediated tyrosine phosphorylation of PLC-γ1 stimulates catalytic activity in vitro9 and G proteins have been implicated in the activation of PLC10, we investigated which PLC isozymes are subject to G protein regulation. We have purified11 an activated G protein α subunit that stimulates partially purified phospholipase C and now report that this G protein specifically activates the β1 isozyme, but not the γ1 and δ1 isozymes of phospholipase C. We also show that this protein is related to the Gq class of G protein α subunits12.

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Author notes

  1. Stephen J. Taylor

    Present address: Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York, 14853, USA

Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tenessee, 37232, USA

    Stephen J. Taylor & John H. Exton

  2. Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Ho Zoon Chae & Sue Goo Rhee

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  2. Ho Zoon Chae
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  3. Sue Goo Rhee
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  4. John H. Exton
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Taylor, S., Chae, H., Rhee, S. et al. Activation of the β1 isozyme of phospholipase C by α subunits of the Gq class of G proteins. Nature 350, 516–518 (1991). https://doi.org/10.1038/350516a0

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