• An Erratum to this article was published on 08 February 2001


With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important to develop reliable proteome-wide approaches for a better understanding of protein function1. As elementary constituents of cellular protein complexes and pathways, protein–protein interactions are key determinants of protein function. Here we have built a large-scale protein–protein interaction map of the human gastric pathogen Helicobacter pylori. We have used a high-throughput strategy of the yeast two-hybrid assay to screen 261 H. pylori proteins against a highly complex library of genome-encoded polypeptides2. Over 1,200 interactions were identified between H. pylori proteins, connecting 46.6% of the proteome. The determination of a reliability score for every single protein–protein interaction and the identification of the actual interacting domains permitted the assignment of unannotated proteins to biological pathways.

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We thank M. Fromont-Racine, P. Glaser, A. Jacquier, A. Brunet and L. Decourty for their help at the launch of this project; M. Fejes, G. Conan and P. Desmoucelle for technical assistance; G. Boissy and J.-L. Divol for their help in software development; F. Colland for his contribution to the mapping of FliA interacting domain on the 3D structure of the core RNA polymerase; and S. Whiteside for a thorough and critical reading of the manuscript. We are very grateful to R. Benarous, J. Camonis, L. Daviet, M. Rosbash, A.D. Strosberg and S. Whiteside for many stimulating discussions. This work was supported by an interest-free loan from the ANVAR. P.L. is on leave from the CNRS.

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  1. Hybrigenics SA, 180 avenue Daumesnil, Paris, 75012, France

    • Jean-Christophe Rain
    • , Luc Selig
    • , Véronique Battaglia
    • , Céline Reverdy
    • , Stéphane Simon
    • , Gerlinde Lenzen
    • , Fabien Petel
    • , Jérôme Wojcik
    • , Vincent Schächter
    • , Y. Chemama
    •  & Pierre Legrain
  2. Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 25 rue du Dr Roux, Paris, 75724 Cedex 15, France

    • Hilde De Reuse
    •  & Agnès Labigne


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Correspondence to Pierre Legrain.

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