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The PSI-H subunit of photosystem I is essential for state transitions in plant photosynthesis

Abstract

Photosynthesis in plants involves two photosystems responsible for converting light energy into redox processes. The photosystems, PSI and PSII, operate largely in series, and therefore their excitation must be balanced in order to optimize photosynthetic performance1. When plants are exposed to illumination favouring either PSII or PSI they can redistribute excitation towards the light-limited photosystem. Long-term changes in illumination lead to changes in photosystem stoichiometry2,3. In contrast, state transition is a dynamic mechanism that enables plants to respond rapidly to changes in illumination. When PSII is favoured (state 2), the redox conditions in the thylakoids change and result in activation of a protein kinase4,5,6. The kinase phosphorylates the main light-harvesting complex (LHCII) and the mobile antenna complex is detached from PSII. It has not been clear if attachment of LHCII to PSI in state 2 is important in state transitions. Here we show that in the absence of a specific PSI subunit, PSI-H, LHCII cannot transfer energy to PSI, and state transitions are impaired.

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Figure 1: Measurements of transitions between state 1 and state 2.
Figure 2: In vitro phosphorylation of LHCII.
Figure 3: Reversible phosphorylation of LHCII in vivo.
Figure 4: Light saturation curve for P700 oxidation in intact leaves.

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Acknowledgements

We thank M. Jensen for technical assistance, B.L. Møller for discussions, and The Danish National Research Foundation for financial support.

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Correspondence to Henrik Vibe Scheller.

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Lunde, C., Jensen, P., Haldrup, A. et al. The PSI-H subunit of photosystem I is essential for state transitions in plant photosynthesis. Nature 408, 613–615 (2000). https://doi.org/10.1038/35046121

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