ClC-5 Cl--channel disruption impairs endocytosis in a mouse model for Dent's disease

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Abstract

Dent's disease is an X-linked disorder associated with the urinary loss of low-molecular-weight proteins, phosphate and calcium, which often leads to kidney stones1,2. It is caused by mutations3 in ClC-5, a renal chloride channel4,5 that is expressed in endosomes of the proximal tubule6,7. Here we show that disruption of the mouse clcn5 gene causes proteinuria by strongly reducing apical proximal tubular endocytosis. Both receptor-mediated and fluid-phase endocytosis are affected, and the internalization of the apical transporters NaPi-2 and NHE3 is slowed. At steady state, however, both proteins are redistributed from the plasma membrane to intracellular vesicles. This may be caused by an increased stimulation of luminal parathyroid hormone (PTH) receptors owing to the observed decreased tubular endocytosis of PTH. The rise in luminal PTH concentration should also stimulate the hydroxylation of 25(OH) vitamin D3 to the active hormone. However, this is counteracted by a urinary loss of the precursor 25(OH) vitamin D3. The balance between these opposing effects, both of which are secondary to the defect in proximal tubular endocytosis, probably determines whether there will be hypercalciuria and kidney stones.

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Figure 1: Generation of clcn5- mice.
Figure 2: Proximal tubular endocytosis with various clcn5 genotypes.
Figure 3: Effect of ClC-5 disruption on transporters and receptors in mice kept on standard diet.
Figure 4: Regulation of NaPi-2 trafficking.
Figure 5: Regulation of NHE3 localization.

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Acknowledgements

We thank G. Boia for technical assistance, P. Gruss for MPI2 ES cells, H. Murer and J. Biber for the NaPi-2 antibody, R. McCluskey for the megalin antibody, S. Gluck and D. K. Stone for proton pump antibodies, B. Blaner for the antibody against retinol binding protein and M. Haddad for chemical analysis of serum and urine samples. This work was supported by grants from the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie, and the Louis-Jeantet Prize for medicine to T.J.J.

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