Brief Communication | Published:

Gene expression

Total silencing by intron-spliced hairpin RNAs

Nature volume 407, pages 319320 (21 September 2000) | Download Citation

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Abstract

Post-transcriptional gene silencing (PTGS), a sequence-specific RNA degradation mechanism inherent in many life-forms, can be induced in plants by transforming them with either antisense1 or co-suppression2 constructs, but typically this results in only a small proportion of silenced individuals. Here we show that gene constructs encoding intron-spliced RNA with a hairpin structure can induce PTGS with almost 100% efficiency when directed against viruses or endogenous genes. These constructs could prove valuable in reverse genetics, genomics, engineering of metabolic pathways and protection against pathogens.

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References

  1. 1.

    , & Nature 346, 284– 287 (1990).

  2. 2.

    Science 268, 689–691 ( 1995).

  3. 3.

    , & Proc. Natl Acad. Sci. USA 95, 13959–13964 (1998).

  4. 4.

    , & Trends Plant Sci. 4, 452– 457 (1999).

  5. 5.

    , , S. & J. Genet. Breed. 51, 325–334 (1997).

  6. 6.

    et al. Plant Cell 6, 147–158 (1994).

  7. 7.

    , , , & Plant Sci. 136, 181– 194 (1998).

  8. 8.

    & Plant Mol. Biol. 29 , 663–678 (1995).

  9. 9.

    Plant Mol. Biol. 20, 1203–1207 (1992).

  10. 10.

    , , & Plant Mol. Biol. 23, 671–683 (1993).

  11. 11.

    et al. Nature 391, 806–811 (1998).

  12. 12.

    Genes Dev. 13, 139–141 ( 1999).

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Author information

Affiliations

  1. CSIRO Plant Industry, Canberra, ACT 2601, Australia

    • Neil A. Smith
    • , Surinder P. Singh
    • , Ming-Bo Wang
    • , Peter A. Stoutjesdijk
    • , Allan G. Green
    •  & Peter M. Waterhouse

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Correspondence to Peter M. Waterhouse.

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DOI

https://doi.org/10.1038/35030305

brief communications is intended to provide a forum for short, topical reports of general scientific interest and for technical discussion of recently published material of particular interest to non-specialist readers. Priority will be given to contributions that have fewer than 500 words, 10 references and only one figure. Detailed guidelines are available on Nature's website (http://www.nature.com) or on request from nature@nature.com

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