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Peptide cyclization catalysed by the thioesterase domain of tyrocidine synthetase

Nature volume 407, pages 215218 (14 September 2000) | Download Citation

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Abstract

In the biosynthesis of many macrocyclic natural products by multidomain megasynthases, a carboxy-terminal thioesterase (TE) domain is involved in cyclization and product release1,2; however, it has not been determined whether TE domains can catalyse macrocyclization (and elongation in the case of symmetric cyclic peptides) independently of upstream domains. The inability to decouple the TE cyclization step from earlier chain assembly steps has precluded determination of TE substrate specificity, which is important for the engineered biosynthesis of new compounds1. Here we report that the excised TE domain from tyrocidine synthetase efficiently catalyses cyclization of a decapeptide-thioester to form the antibiotic tyrocidine A, and can catalyse pentapeptide-thioester dimerization followed by cyclization to form the antibiotic gramicidin S. By systematically varying the decapeptide-thioester substrate and comparing cyclization rates, we also show that only two residues (one near each end of the decapeptide) are critical for cyclization. This specificity profile indicates that the tyrocidine synthetase TE, and by analogy many other TE domains, will be able to cyclize and release a broad range of new substrates and products produced by engineered enzymatic assembly lines.

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Acknowledgements

This work was supported by grants from the NIH (to C.T.W.), the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie (to M.A.M.), a NIH postdoctoral fellowship (to J.W.T.) and a PhD fellowship of the Stiftung Stipendien-Fonds des Verbandes der Chemischen Industrie (to H.D.M.).

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  1. *Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA

    • John W. Trauger
    • , Rahul M. Kohli
    •  & Christopher T. Walsh
  2. †Biochemie/Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany

    • Henning D. Mootz
    •  & Mohamed A. Marahiel

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Correspondence to Christopher T. Walsh.

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https://doi.org/10.1038/35025116

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