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WNT signalling molecules act in axis formation in the diploblastic metazoan Hydra

Nature volume 407, pages 186189 (14 September 2000) | Download Citation



Members of the Wnt/wingless family of secreted proteins act as short-range inducers and long-range organizers during axis formation, organogenesis and tumorigenesis in many developing tissues1. Wnt signalling pathways are conserved in nematodes, insects and vertebrates2. Despite its developmental significance, the evolutionary origin of Wnt signalling is unclear. Here we describe the molecular characterization of members of the Wnt signalling pathway—Wnt, Dishevelled, GSK3, β-Catenin and Tcf/Lef—in Hydra, a member of the evolutionarily old metazoan phylum Cnidaria. Wnt and Tcf are expressed in the putative Hydra head organizer, the upper part of the hypostome. Wnt, β-Catenin and Tcf are transcriptionally upregulated when head organizers are established early in bud formation and head regeneration. Wnt and Tcf expression domains also define head organizers created by de novo pattern formation in aggregates. Our results indicate that Wnt signalling may be involved in axis formation in Hydra and support the idea that it was central in the evolution of axial differentiation in early multicellular animals.

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  1. 1.

    & Wnt signalling: a common theme in animal development. Genes Dev. 11, 3286– 3305 (1997).

  2. 2.

    & Wingless signalling: The inconvenient complexities of life. Curr. Biol. 8, R140 –R144 (1998).

  3. 3.

    et al. Identification of a hydra homologue of the β-catenin/plakoglobin/armadillo gene family. Gene 172, 155– 159 (1996).

  4. 4.

    et al. Identification and characterization of the epithelial polarity receptor “Frizzled” in Hydra vulgaris. Dev. Genes Evol. 210, 258–262 (2000).

  5. 5.

    et al. XTcf-3 transcription factor mediates β-catenin-induced axis formation in Xenopus embryos. Cell 86, 391–399 (1996).

  6. 6.

    & in Pattern Formation. A Primer in Developmental Biology (eds Malacinski, G. M. & Bryant, S. V.) 213–241 (Macmillan, New York, 1984 ).

  7. 7.

    A model for pattern formation of hypostome, tentacles, and foot in hydra: How to form structures close to each other, how to form them at a distance. Dev. Biol. 157, 321–333 (1993).

  8. 8.

    Principles of Development (Oxford Univ. Press, Oxford, 1998).

  9. 9.

    & Synexpression groups in eukaryotes. Nature 402, 483–487 (1999).

  10. 10.

    & Budding in Hydra attenuata : Bud stages and fate map. J. Exp. Zool. 200, 417–428 (1977).

  11. 11.

    & Genetic analysis of developmental mechanisms in Hydra. X. Morphogenetic potentials of a regeneration-deficient strain (reg-16). Dev. Biol. 107, 13– 27 (1985).

  12. 12.

    et al. Regeneration of hydra from reaggregated cells. Nature New Biol. 239, 98–101 (1972).

  13. 13.

    & A theory of biological pattern formation. Kybernetik 12, 30– 39 (1972).

  14. 14.

    Hydra transplantation phenomena and the mechanism of Hydra head regeneration. II. Properties of head activation. Dev. Biol. 96, 239–257 (1983).

  15. 15.

    et al. Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. Cell 88, 789–799 ( 1997).

  16. 16.

    & Expression of wingless in the Drosophila embryo: a conserved cis-acting element lacking conserved Ci-binding sites is required for patched-mediated repression. Development 125, 1469–1476 (1998).

  17. 17.

    Metazoan phylogenies: falling into place or falling to pieces? A palaeontological perspective. Curr. Op. Genet. Dev. 8, 662 –667 (1998).

  18. 18.

    A. et al. Evidence for a clade of nematodes, arthropods and other moulting animals. Nature 387, 489–493 (1997).

  19. 19.

    & Early animal evolution: Emerging views from comparative biology and geology. Science 284, 2129–2137 (1999).

  20. 20.

    , & The zootype and the phylotypic stage. Nature 361, 490–492 ( 1993).

  21. 21.

    & The evolution of the Hox cluster: insights from outgroups. Curr. Op. Genet. Dev. 8, 681–687 ( 1998).

  22. 22.

    , & Isolation of Hox genes from the scyphozoan Cassiopeia xamachana: Implications for the early evolution of Hox genes. Mol. Dev. Evol. 285, 63–75 (1999).

  23. 23.

    et al. Evolution of Antp-class genes and differential expression of Hydra Hox/paraHox genes in anterior patterning. Proc. Natl Acad. Sci. USA 97, 4493– 4498 (2000).

  24. 24.

    & Long-range action of Wingless organizes the dorsal-ventral axis of the Drosophila wing. Development 124, 871–880 (1997).

  25. 25.

    , & Wingless, Decapentaplegic and EGF receptor signalling pathways interact to specify dorso-ventral pattern in the adult abdomen of Drosophila. Development 126, 3495– 3507 (1999).

  26. 26.

    et al. Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in β-catenin that are modulated by the Wnt signalling pathway. J. Cell Biol. 136, 1123–1136 (1997).

  27. 27.

    Invertebrate Relationships. Patterns in Animal Evolution (Cambridge Univ. Press, Cambridge, UK, 1990).

  28. 28.

    Animal Evolution: Interrelationships of the Living Phyla (Oxford Univ. Press, Oxford, 1995).

  29. 29.

    in PCR Primer—A Laboratory Manual (eds Dieffenbach, C. W. & Dveksler, G. S.) 381–409 (CSHL, New York, 1995).

  30. 30.

    & HyBra1, a brachyury homologue, acts during head formation in hydra. Development 126 , 999–1010 (1999).

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We thank M. Sarras for sharing unpublished data, the Mishima Hydra lab for providing reg-16 mutants, C. N. David and U. Technau for critical comments on the manuscript, and K. Wehner and P. Lübberich for assistance in cloning. This work was supported by grants from the Deutsche Forschungsgemeinschaft (B.H. and T.W.H).

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  1. *Department of Molecular Cell Biology, Zoological Institute, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany

    • Bert Hobmayer
    • , Fabian Rentzsch
    • , Kerstin Kuhn
    • , Christoph M. Happel
    • , Christoph Cramer von Laue
    • , Petra Snyder
    •  & Thomas W. Holstein
  2. †Laboratoire de Genetique et Physiologie du Developpement, IBDM, Campus de Luminy, 13288 Marseille Cedex 9, France

    • Ute Rothbächer


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Correspondence to Bert Hobmayer.

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